Influence of Stroke on the Composition of Intestinal Microbiota

N/ACompletedNCT02008604

Charite University, Berlin, Germany24 enrolled

Overview

The aim of the study is to verify the hypothesis that the microbial colonisation of the gut is changed in patients after stroke and that the gut microbiome of severely affected stroke patients differs from that of patients with only a short disruption of blood circulation in the brain (transient ischemic attack, TIA). For this, the composition of gut microbiota in stool samples will be analyzed by 454 pyrosequencing. Further, the correlation of stroke-associated changes in the microbiome with immunological parameters will be analyzed.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Stroke Transient Ischemic Attack (TIA)

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * age: 18 and older * ischemic cerebral infarction within the last 40 hours, anterior circulation infarct (ACA, MCA) and/or posterior circulation infarct (PCA, BA) of any severity * patient consent or consent by a legal representative * NIHSS \> or =9 (stroke patients) * NIHSS \< 1 (for TIA patients) Exclusion Criteria: * intracranial hemorrhage * preexisting dysphagia * signs of infection upon admission (clinical/paraclinical) * existing antibiotic therapy upon admission or within the last 2 weeks before admission * mechanical ventilation upon admission * participation in an interventional therapy study

Locations (1)

Charite Universitätsmedizin Berlin

Berlin, Germany

Outcomes

Primary Outcomes

composition of intestinal microbiome

The composition of the intestinal metagenome / microbiome will be determined by analyzing the 16S rRNA by 454 Pyrosequencing.

Time frame: change between day 1, day 4, day 7, day 14 after stroke

Secondary Outcomes

test of correlation between most changed group of bacteria with changes in immunological parameters (HLA-DR)

Analysis of correlation between changes in microbiome (most changed group of bacteria, e.g. lactobacilli) with changes in immunological parameters (e.g. HLA-DR, IL-6, IL-10, CD4-lymphocytes from blood samples).

Time frame: days 1, 4, 7 after stroke

Data from ClinicalTrials.gov

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