In this study healthy older adults are requested to consume 4 different high-protein nutritional supplements. Each subject will visit the site 4 times and at every visit they will consume 1 of the 4 products after which a series of blood samples will be taken. The blood samples will be analyzed for amino acid bioavailability in the blood up to 4 hours after consumption.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
12
High-whey protein, leucine-rich, low-caloric ONS in powder format
High casein-protein, low-caloric control product (isocaloric to product A)
High casein-protein, high-caloric control product.
Ampha
Nijmegen, Netherlands
The primary outcome parameter in this study is the Maximum serum leucine concentration (Leumax) [micromol/L].
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Serum leucine incremental area under the curve (iAUC) [micromol/L*min].
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Time to reach half the serum leucine iAUC (t½) [minutes].
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Maximum serum essential amino acid concentration (EAAmax) [micromol/L]
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Maximum serum amino acid concentration (AAmax) [mmol/L]
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Maximum serum insulin concentration [micromol/L].
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Serum insulin iAUC [micromol/L*min]
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Maximum serum glucose concentration [mmol/L]
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Serum glucose iAUC [mmol/L*min]
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high whey-protein, leucine-rich, high-caloric control product (isocaloric to product C)
Time frame: Up to 4 hours after each bolus intake (4 visits, 7-10 days between 2 consecutive visits)
Adverse events and (Gastro-Intestinal) tolerance questionnaire.
Time frame: During the study and until one week after completion of the study