In the present pilot study, the investigators will study the effects of a novel synbiotic, which is a mix of probiotics (Ecologic 825) in the presence of a prebiotic (short chain fructo-oligosaccharide (scFOS)), on mucosal integrity, overall microbiota changes along the gastrointestinal-tract and the mucosal immune response. The investigators hypothesize that the synbiotic Ecologic 825/scFOS will significantly affect the intestinal permeability and modulate the immune system in humans.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
QUADRUPLE
Enrollment
20
Maastricht University Medical Center
Maastricht, Limburg, Netherlands
Sugar recovery in urine, as indicator of intestinal permeability
Time frame: Baseline and after 2 weeks intervention
Biomarkers of immune modulation; plasma levels of Tumor necrosis factor alpha , Interleukin-1 beta, Interleukin-6, Interleukin-8, Interleukin-17, Monocyte chemoattractant protein-1 and Macrophage inflammatory protein-1a
Time frame: Baseline and after 2 weeks intervention
Relative abundance of commensal rat ileum bacterium (CRIB) by quantitative polymerase chain reaction (qPCR)
Time frame: Baseline and after 2 weeks intervention
Barcoded pyrosequencing of 16S rRNA genes of the microbiota
Time frame: Baseline and after 2 weeks intervention
Metatranscriptomics of the microbiota
Time frame: Baseline and after 2 weeks intervention
10 item gastrointestinal symptom diary
Time frame: Baseline and after 2 weeks intervention
Quantitative Insights Into Microbial Ecology (QIIME) of the microbiota
Time frame: Baseline and after 2 weeks intervention
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