This trial is an exploratory study in order to determine whether the ingestion of one type of protein (milk protein/CHO/Fat) vs.placebo (CHO/Fat) 2 hours before bed time results in the extension of the post-prandial window of anabolism during the night by increasing whole body protein balance as well as muscle protein fractional synthesis rate (FSR).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
17
Study product will provide 25 g of protein to subjects.
Product with no protein
Metabolic Unit
Lausanne, Canton of Vaud, Switzerland
muscle FSR determination
Muscle protein FSR (fractional synthesis rate) will be determined using primed constant infusions of \[13C6\] phenylalanine for 5 hours starting around from 03:00 a.m. (see flow chart attached). Whole body protein balance will be measured throughout the night using \[15N\]glycine (200mg) which will be orally taken with the trial product in the evening.
Time frame: Within 24 hours of product intake
Activation of signaling pathways involved in skeletal muscle protein balance
Activation of signaling pathways involved in skeletal muscle protein balance will be assessed through use of immunohistochemistry
Time frame: Within 24 hours after product intake
Glucose metabolism
Glucose metabolism will be assessed from circulating concentrations of glucose and insulin.
Time frame: Within 24 hours after product intake
Plasma amino acid availability
The quantification of Free Amino Acids in Human Plasma will be done by Amino Acid Analyser Biochrome 30.
Time frame: Within 24 hours after product intake
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