The main objective of this study is to characterize the evolution (over a period of 28 days) of superficial and deep bacterial skin flora in patients with stage 3 or 4 sacral pressure ulcers (bedsores). Changes in terms of pathogenic, potentially pathogenic and non-pathogenic bacterial species at the intra-individual and population levels.
The secondary objectives of this study are: A. To compare bacterial communities collected from superficial versus deep samples of stage 3 or 4 sacral bedsores using metagenomics and standard culturing methods. B. To characterize the evolution of digestive bacterial flora in the same patients over a period of 28 days. Changes in terms of pathogenic, potentially pathogenic and non-pathogenic will be described, as well as the appearance of resistance phenotypes. C. To characterize the evolution of urinary bacterial flora in the same patients over a period of 28 days. Changes in terms of pathogenic, potentially pathogenic and non-pathogenic will be described, as well as the appearance of resistance phenotypes.
Study Type
OBSERVATIONAL
Enrollment
55
Bacterial samples will be collected using deep level tissue biopsy using a Steifel punch of 3 mm in diameter (days 0 and 28).
Bacterial samples will be taken from the surface of the bedsore via swabbing. (days 0 \& 28)
Stool samples will be collected on days 0 and 28.
Centre Mutualiste Neurologique Propara
Montpellier, France
Laboratoire d'Etude et de Recherche en Environnement et Santé, Unité Biodiagnostic, UNIMES
Nîmes, France
CHRU de Nîmes - Hôpital Universitaire Carémeau
Nîmes, France
Unité U1047 "Bacterial virulence and infectious diseases", UFR de Médecine
Nîmes, France
List of pathogenic, potentially pathogenic and commensal bacterial species in superficial and deep bedsore samples
At the individual level
Time frame: Baseline (day 0)
List of pathogenic, potentially pathogenic and commensal bacterial species in superficial and deep bedsore samples
At the individual level
Time frame: Day 28
Percentage of pathogenic, potentially pathogenic, and commensal bacterial species in superficial and deep bedsore samples
Calculated over the entire study population
Time frame: baseline (day 0)
Percentage of pathogenic, potentially pathogenic, and commensal bacterial species in superficial and deep bedsore samples
Calculated over the entire study population
Time frame: Day 28
Classification of bacteria present according to respiratory type, gram negative/positive, shape, and resistance phenotypes.
Based on superficial and deep bedsore samples.
Time frame: baseline (Day 0)
Classification of bacteria present according to respiratory type, gram negative/positive, shape, and resistance phenotypes.
Based on superficial and deep bedsore samples.
Time frame: Day 28
List of pathogenic, potentially pathogenic and commensal bacterial species in stool samples
Time frame: baseline (day 0)
List of pathogenic, potentially pathogenic and commensal bacterial species in stool samples
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Urine samples will be collected on days 0 and 28.
Time frame: Day 28
List of pathogenic, potentially pathogenic and commensal bacterial species in urine samples
Time frame: baseline (day 0)
List of pathogenic, potentially pathogenic and commensal bacterial species in urine samples
Time frame: day 28