Modulation of CSF Amyloid-beta Concentrations Via Behavioral Sleep Deprivation and Pharmacological Sleep Induction

Inclusion Criteria: * cognitively normal or CDR 0 * negative for amyloid deposition by PET Pittsburgh Compound-B (PIB) imaging or CSF amyloid-beta-42 concentration * Age 18-60 * Average reported sleep time 6-10hrs Exclusion Criteria: * diagnosis of a sleep disorder such as sleep apnea, narcolepsy, or restless leg syndrome * positive ambulatory sleep study for obstructive sleep apnea (AHI \> 5 respiratory events per hour) that will be performed as part of initial screening prior to enrollment * Clinical Dementia Rating (CDR) \> 0 * tremor or other neurologic injury in the non-dominant upper extremity (such as stroke or tremor) that would prevent the use of actigraphy * current sleep walking or other sleep parasomnia * diagnosis and treatment of stroke, myocardial infarction or heart attack, * coronary artery disease, atrial fibrillation, or congestive heart failure * diagnosis and treatment of asthma or Chronic Obstructive Pulmonary Disease (COPD) * diagnosis and treatment of bipolar disorder, major depression, or Schizophrenia * current urinary or fecal incontinence * currently on a low salt diet * diagnosis and treatment of a neurologic disorder such as Parkinson's disease, epilepsy, multiple sclerosis * currently taking any blood thinner medications such as warfarin, Plavix, or Aspirin * kidney disease resulting in renal impairment * liver disease resulting in hepatic dysfunction * Pregnancy * currently taking sedating medications such as benzodiazepines * alcohol use at bedtime * tobacco use * BMI \>40 * contraindication to lumbar puncture * diabetes * sleep schedule outside the range of bedtime: 8pm-12am and waketime 4am-8am * self reported difficulty sleeping in an unfamiliar environment * use of sedative-hypnotic medications * inability to get in and out of bed * history or presence of any clinically significant medical condition, behavioral or psychiatric disorder, or surgical history based on medical record or patient report * history of drug abuse within the past 6 months * positive score on 2 or more categories on the Berlin questionnaire * participation in another investigational medicinal product or investigational device within the last 30 days