The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.
This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media: 1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult) 2. P1 medium vs. ECM medium (Irvine). Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.
Study Type
OBSERVATIONAL
Enrollment
600
Hillel Yaffe Medical Center
Hadera, Israel
Fertilization Rate
Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated
Time frame: 2 years
Embryo Quality
Embryo quality and developmental rate will be retrospectively compared according to culture media in which sibling oocytes were incubated
Time frame: 2 years
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