Diagnosis of Periodontal Disease Using Genetic Marker in Saliva

Inclusion Criteria: A. Severe Periodontal Disease * Patients with chronic severe periodontitis * Primary treatment of periodontal scaling, subgingival curettage is required, and the general progress of the protocol agreed with the basic periodontal therapy patients. * subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing A. Moderate Periodontal Disease * Patients with chronic moderate periodontitis * Primary treatment of periodontal scaling, subgingival curettage is required, and the general progress of the protocol agreed with the basic periodontal therapy patients. * subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing C. Healthy Control * Healthy periodontal patients ( more than 20 permanent teeth are present , in all parts of deep periodontal pockets measuring 4mm or less, and height is 2mm or less clinical attachment person) * subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing Exclusion Criteria: * Received periodontal treatment within the last six months * recently taken antibiotics within 3 months if you need a prescription antibiotic prophylaxis * taking the drug can affect periodontal status for a long period of time * pregnant or breastfeeding * In case of oral inflammatory mucosal disease (Lichen planus, etc.) * A history of alcoholism or drug abuse if * other special systemic disease (high blood pressure) * ages under 18