Association of CYP3A4 rs2242480 With Sufentanil Plasma Concentration and PCA Consumption

Overview

This study was designed for exploring the association of cytochrome P450 3A4 rs2242480 polymorphism with metabolism of sufentanil in Chinese patients receiving upper abdominal surgery，to provide evidence for genetic prediction of personalized medication.Methods:60 patients who prepared for elective upper abdominal surgery under general anesthesia were recruited into study. Liver enzyme inhibitor should be excluded in anesthesia. rs2242480 genotyping was carried out by direct sequencing. The blood samples were drawn before anesthesia，30 min and 45min after anesthesia respectively，used for plasma sufentanil concentration detection with high performance liquid chromatography-mass spectrometry (HPLC-MS). After surgery，the patients received patient-controlled intravenous sufentanil immediately. And postoperative pain at rest，sufentanil consumption，side effects and rescue analgesic requirements were recorded at the 6th，12th，24th hour. The plasma sufentanil concentration and PCA sufentanil consumption were analyzed to investigate the differences among rs2242480 three genotypes.

The study consisted of healthy men and women between 18 to 65 years of age. Participants were screened and stratified according to cytochrome P450 3A4 rs2242480 genotype: * 1/\*1 Grouped by rs2242480 polymorphism, wild-type homozygote * 1/\*1G Grouped by rs2242480 polymorphism,\*1/\*1G: mutant heterozygote * 1G/\*1G Grouped by rs2242480 polymorphism,\*1G/\*1G: mutant homozygote

Conditions

Eligibility