Accelerated Immunosenescence and Chronic Kidney Disease

Centre Hospitalier Universitaire de Besancon222 enrolled

Overview

The aim of this study is to investigate the impact of renal function and dialysis techniques on the percentage of senescent T lymphocytes.

The immunosenescence is a complex and profound remodeling of the immune system during life. It is mainly due to thymic involution and repeated antigenic stimulation. Kidney disease is associated with a decrease in adaptive immunity as evidenced by the decrease in vaccine response and increased susceptibility to infections, similar to those observed in the elderly population. However, data on aging of the immune system in chronic kidney disease remains incomplete. Furthermore, the determinants of immunosenescence are not also not known. It is possible that "uremic" factors help explain the phenotypic and functional changes of lymphocytes, as antigenic stimuli associated with repeated bio-compatible materials used in dialysis contact. The purpose of this study is to describe the phenotypes of the immune system of renal and analyze the determinants of these changes.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

OTHER

Masking

NONE

Enrollment

222

Conditions

Renal Failure

Interventions

Blood sampleBIOLOGICAL

3 tubes of 5 ml tubes and 4 of 7 ml for biological assays at t0.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 85 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient able to understand the reason of the study * Patient not opposed to the conservation of biological samples for scientific research Exclusion Criteria: * Patient suffering from psychotic illness * Any history of immunosuppressive therapy (except steroids up to 5mg/day) * History of cancer (except skin cancer) or treated hematological malignancy * Infectious episode required hospitalization not older 3 months * Hepatitis B or C infection * HIV infection, active or inactive * For dialysis patients: renal failure on dialysis for less than 3 months and/or have benefited from two techniques for renal replacement therapy in the last 6 months

Locations (1)

Service de néphrologie, CHU de Besançon

Besançon, France

Outcomes

Primary Outcomes

Percentage of Cluster of Differentiation (CD) 4/8+ CD 57+ CD 28- lymphocytes (senescent lymphocytes)

The primary outcome measure is the percentage of CD 4/8+ CD 57+ CD 28- lymphocytes by flow cytometry. The technique used is a 6 colors surface labelling of T lymphocytes to study the T cell senescent population.

Time frame: 6 months

Secondary Outcomes

Telomerase Activity of T lymphocytes

The telomerase activity of T lymphocytes is assessed by a method of Polymerase chain reaction (PCR)-ELISA.

Time frame: 6 months

Level of phospho-histone 2AX (gH2AX) in peripheral blood T lymphocytes

This level is obtained by flow cytometry after permeabilization and labelling of Peripheral Blood Mononuclear Cells (PBMC).

Time frame: 6 months

Proportion of Recent Thymic Emigrants (RTE) in peripheral blood T lymphocytes

RTE T cells will be defined by flow cytometry, according to co-expression of CD 4, CD 8,CD 31 and CD 45RA

Time frame: 6 months

T-cell receptor excision circle (TREC) level in PBMC.

TRECs study is based on a technique of quantitative PCR using DNA extracted from PBMC.

Time frame: 6 months

Telomere length in T lymphocytes

Study of telomere length is performed after extraction of DNA from isolated T cells. The length is determined by a quantitative PCR technique relative to a reference gene.

Time frame: 6 months

Data from ClinicalTrials.gov

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