Changes in Tumor Tissue and Serum Biomarkers Before and After Cetuximab Combined With Preoperative Radiotherapy in Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma

Inclusion Criteria: (1)18 to 65 years old,male or female (2)Pathologically or cytologically proven thoracic middle-lower esophageal squamous cell carcinoma; (3)clinical stage II or stage III; (4)Primary tumors can be measured; (5)Karnofsky score \>70; (6)Estimated life expectancy of at least 12 weeks; (7)reproductive age women should ensure that before entering the study period contraception; (8)Hemoglobin≥90.0g/dL,white blood cell count（WBC）≥ 4000 cells/mm³,Platelet count≥100,000 cells/mm³; (9)Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) \< 2.5 times upper limit of normal,bilirubin normal; (10)Creatinine normal OR creatinine clearance ≥ 60 mL/min; (11)Patients have good compliance to treatment and follow-up of acceptance; (12)Forced expiratory volume in one second（FEV1） ≥ 1.5 litre or ≥ 75% of the reference value; (13)Considered operable (i.e. appropriate organ functions and ability to undergo general anesthesia); (14)No severe or uncontrolled cardiovascular disease; (15)No active uncontrolled infection; Exclusion Criteria: 1. Patient who have distant metastasis 2. The primary tumor or lymph node already received surgical treatment (except for biopsy); 3. Patient who received radiotherapy for primary tumor or lymph node; 4. Patient who received the the epidermal growth factor targeted therapy; 5. Patient who received chemotherapy or immunotherapy; 6. Patient who suffered from other malignant tumor; 7. Epidermal growth factor receptor（EGFR）mutation（-）; 8. Patient who have taken other drug test within 1 month; 9. Pregnant woman or Lactating Women and Women in productive age who refuse to take contraception in observation period; 10. Subject with a severe allergic history or idiosyncratic; 11. Subject with severe pulmonary and cardiopathic disease history; Refuse or incapable to sign the informed consent form of participating this trial; 12. Drug abuse or alcohol addicted; 13. Subject with a Personality or psychiatric diseases, people with no legal; capacity or people with limited capacity for civil conduct;