The investigators hypothesis: Presence of anti-GGT (antibody against GGT) indicates H. pylori infection.
Subjects will be requested to provide 1-5g of their solid/semi-solid stool sample, to be collected within 24 hours prior to their scheduled visit for urea breath test. Patients should not have been treated for H. pylori prior to stool collection. Stool samples will be tested using ELISA for the presence of the H. pylori GGT antigen using monoclonal antibodies against GGT. Stool samples will also be tested for the presence of H. pylori DNA using PCR and Western blot analysis respectively. Presence of the GGT antigen in stool will be compared between H. pylori-positive and H. pylori-negative patients. Rapid urease test results will be used as the gold standard of H. pylori diagnosis. The sensitivity and specificity of the stool antigen test will be established.
Study Type
OBSERVATIONAL
Enrollment
100
Nuhs Umc/Udc
Singapore, Singapore, Singapore
RECRUITINGStool anti-GGT for the diagnosis of Helicobactor Pylori Infection
1-5g of stool samples will also be collected from patients recruited to this study, in a preliminary investigation to determine the sensitivity and specificity of a stool H.pylori antigen test. Rapid urese test is the gold standard.
Time frame: 1 month after stool samples collected
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