The main objectives of the study are: To determine if RNA recovery from tape harvesting allows for the identification of a disease gene signature (e.g., interferon \[IFN\] signature for lupus) or other biomarkers that may differentiate affected from normal or unaffected skin; To determine if the lupus gene signature is differentially expressed in the epidermis from active discoid lupus erythematosus (DLE) or subacute cutaneous lupus erythematosus (SCLE) lesions when compared with unaffected skin from the same participants and from the skin of healthy volunteers (HVs); To determine if the atopic dermatitis (AD) gene signature is differentially expressed in the epidermis from active AD lesions when compared with unaffected skin from the same participants and from the skin of HVs; and To correlate the levels of transcripts of targeted genes in the skin by tape harvesting with those obtained from the blood.
No study drug is administered as part of this study. All participants except healthy volunteers will be treated according to standard clinical practice.
Study Type
OBSERVATIONAL
Enrollment
37
Skin taping from affected and unaffected sites (4 tape harvestings/area plus photograph of taping site)
Sampling for biomarker and basic research in CLE and AD
As described in the treatment arm
Research Site
Boston, Massachusetts, United States
Research Site
Boston, Massachusetts, United States
RNA expression of genes suspected to be associated with skin disease in affected versus unaffected skin in participants with skin disease
Participants with skin disease include participants affected with cutaneous lupus erythematosus (\[CLE\] i.e., DLE or SCLE), SCLE, AD
Time frame: Day 1
RNA expression of genes suspected to be associated with skin disease in healthy skin from HVs versus affected skin from participants with skin disease
Time frame: Day 1
Comparison of RNA expression of genes suspected to be associated with skin disease in healthy skin from HVs versus unaffected skin from participants with skin disease
Time frame: Day 1
Correlation between expression levels obtained from tape harvesting and those obtained from blood samples for each identified gene
Time frame: Day 1
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