The Implementation of Novel 'Heavy' Water Techniques for Determining Modulation of Muscle Protein, DNA and Lipid Synthesis Due to Ageing

University of Nottingham20 enrolled

Overview

Maintenance of body's skeletal muscle is key to a healthy older age. However, as we age we lose on average 1-2% of our muscle each year. In order to slow this loss we need to understand fully the mechanisms regulating muscle mass with ageing. In this project we aim to determine these mechanisms using a new novel technique of 'heavy' water ingestion, which will allow us to measure multiple aspects of skeletal muscle mass control during normal everyday activities in young and old individuals over a period of 6 weeks. We will also determine the influence of resistance exercise training during this period in offsetting declines in muscle with ageing using this method

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

PREVENTION

Masking

NONE

Enrollment

Conditions

Healthy

Interventions

Eligibility

Sex: MALEMin age: 18 YearsMax age: 75 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Young (18-30y) and old men (65-75y) who are generally healthy and recreationally active Exclusion Criteria: * Active cardiovascular disease * Cerebrovascular disease including previous stroke, aneurysm (large vessel or intracranial) * Respiratory disease including pulmonary hypertension or COPD * Hyper/ hypo parathyroidism, hyper/ hypothyroidism, Cushing's disease, diabetes * Active inflammatory bowel disease * Renal disease * Malignancy * Recent steroid treatment (within 6 mo), or hormone replacement therapy * Clotting dysfunction * Musculoskeletal or neurological disorders * Any disease requiring long-term drug prescriptions, including statins

Outcomes

Primary Outcomes

Muscle Protein Synthesis

Comparison of muscle protein synthesis between young and older individuals and in response to 6 weeks unilateral exercise training

Time frame: 0-6 Weeks

Secondary Outcomes

Muscle Sub fractions

Comparison of different sub fractions involved in muscle mass regulation between young and older individuals and in response unilateral exercise training

Time frame: 0-6 week