Mechanisms and Factors Responsible for the Inhibition of Transposons During Fetal Gonad Development in Humans

Centre Hospitalier Universitaire Dijon30 enrolled

Overview

This is a single-centre study over 36 months for the inclusion of 30 patients. The selection and inclusion of patients will take place according to the criterion of Medical Termination of Pregnancy at 4 different gestational ages (8-12 weeks of gestation (WG)), 13-16 WG, 17-23 WG and 25-35 WG. The tissue analysed will principally be the gonads, other somatic tissue will also be systematically harvested in parallel (liver and psoas muscle) for control purposes. The gonads will be subjected to molecular biology analyses destined to quantify the expression of transposons and their inhibitors.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Medical Termination of Pregnancy Voluntary Termination of Pregnancy

Interventions

surgical biopsiesOTHER

Eligibility

Medical Language ↔ Plain English

Inclusion Criteria: * Couple or mother who has provided written informed consent * Foetus between 10 and 37 weeks of amenorrhea (WA) * Male foetus * Foetus obtained following medical termination of pregnancy or voluntary termination of pregnancy Exclusion Criteria: * persons without national health insurance cover * foetus presenting aneuploidy * foetus presenting a malformation of the genital organs

Outcomes

Primary Outcomes

Spatio-temporal analysis of the activity of TEs by RT-qPCR and immunostaining

Time frame: Baselines

Secondary Outcomes

Promoter methylation profile by pyrosequencing