The aim of this study is divided in 4 different steps: * the first step has two different purpose: assess the impact of the storage medium of fresh prostatic chips on fluorescence signal and adjust the entire chain (immunolabelling,counter-stain and imaging), * the second step is the adaptation of immunolabelling protocol on histopathology slides, using fresh prostatic tissue, * the third step is to validate the use of: 1. the medical device created by the FEMTO-ST institute use for the detection of fluorescence signal on fresh tissue, 2. the Light-CT scanner use for tissue structural observation. * the four step is to check the preservation of morphological structure of tissue under the effect of laser excitation from the medical device.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
5
All subjects of the study are going to have a trans-urethral resection of prostate.
The FEMTO-ST institute medical device is use to detect prostatic and malignant tissue.
The Light-CT Scanner is use in the tissue structural observation
Grenoble University Hospital, Urology and Anatomopathology Department
Grenoble, France
Complete fluorescence measurement over each field of vision.
Determinate for the anti-PSMA antibody with Alexa Fluor 488 tracer, the ideal couple of antibody concentration/incubation time giving the highest immunofluorescent signal in the adjustment of fixed tissue sample protocol to fresh tissue sample protocol.
Time frame: 1 day
Complete fluorescence measurement over each field of vision.
Determinate for the anti-PSMA antibody with R-Phycoerythrin tracer, the ideal couple of antibody concentration/incubation time giving the highest immunofluorescent signal in the adjustment of fixed tissue sample protocol to fresh tissue sample protocol.
Time frame: 1 day
Quality criteria of the structure of tissue (ordinal qualitative variables).
Verification of the preservation of morphological structure of tissue under the effect of laser excitation from the medical device.
Time frame: 1 day
Fluorescence measurements mean.
Validate the medical device from "FEMTO-ST institute" for the detection of fluorescence signals on fresh prostatic tissue.
Time frame: 1 day
Comparison of prostatic chips tissue architecture between the images from the Light-CT scanner and standard histopathology slides
Validate the Light-CT scanner from LLTech laboratory in the fresh prostatic tissue structural observation
Time frame: 1 day
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