The two specific aims of this study are: 1) to assess the impact of a genetic variant on iron status; 2) to assess the impact of a genetic variant on non-heme absorption among Asian women. The investigators hypothesize that the genetic variant could enhance iron status and iron absorption in Asian women.
To determine the HFE genotype of female Asian study volunteers, we collected venous blood samples and extracted DNA from these blood samples. HFE genotype was determined as CC, CT, and TT and used to assess possible differences in iron status as a function of genotype. To measure the impact of genotype on non-heme Fe absorption, a sub-group of women with the CC (n=10) or TT (n=11) genotype were invited to return for an Fe absorption study. Each volunteer consumed stable 57Fe (as ferrous sulfate). Two weeks after ingesting this tracer, a blood sample was collected from each woman and the amount of non-heme (57Fe) iron incorporated into red blood cells was measured with magnetic sector thermal ionization mass spectrometry. Possible associations between iron status and iron absorption as a function of genotypes were explored. Data from this study will provide information that aims to improve human health by better understanding the iron requirements of individuals with different genetic background.
Study Type
OBSERVATIONAL
Enrollment
57
Human Metabolic Research Unit, Cornell University
Ithaca, New York, United States
Genotype at SNP rs9366637
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining genotype.
Time frame: 2 weeks after the participant's visit to the laboratory
Hemoglobin
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of hemoglobin.
Time frame: Up to 2 days after the participant's visit to the laboratory
Serum Ferritin
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum ferritin.
Time frame: Up to 6 months after the participant's visit to the laboratory
Serum transferrin receptor
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum transferrin receptor.
Time frame: Up to 6 months after the participant's visit to the laboratory
Non-heme Iron absorption in women with 2 HFE genotypes
Women donated a blood sample for genotyping. Women with particular HFE genotypes were invited to return for an absorption study. Two weeks after women consume the iron tracer, a blood sample was obtained to measure the amount of iron absorbed.
Time frame: one month for screening with genotype then 2 weeks for iron absorption study
Folate
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of folate.
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Time frame: Up to 6 months after the participant's visit to the laboratory
Vitamin B-12
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of vitamin B-12.
Time frame: Up to 6 months after the participant's visit to the laboratory
C-reactive protein
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of C-reactive protein.
Time frame: Up to 6 months after the participant's visit to the laboratory