This study will provide the researchers with an opportunity for the investigation of the biomarkers and different human biology component variable interactions in children; information that can generate new correlations between markers not known at the moment. Regarding their analysis, variation could have multiple connotations. A categorical that refers to a specific state, established with a diagnostic standard. And a numerical, referring to a range or the absolute value limits in a continuous spectrum; this research will allow us to express variability in the distance amplitude of the obtained values level, as well as classifications or categories previously established in the possible cases. OBJECTIVES PRINCIPAL OBJECTIVE To estimate the inter and intra-individual variety in a selected series or biological variables determinant of growth, body composition, infection, oxidation, inflammation and hydration, in preschool children sharing the same institutional-based diet. SPECIFIC OBJECTIVES * To determine the inter and intra-individual variation of growth in preschool children, with a similar diet, who attend Secretariat of Beneficial Works of the First Lady system daycare centers and to compare between semi-urban and rural. * To describe the inter-individual variation of the red blood cells circulating mass in preschool children, with a similar diet, who attend day care centers and to compare between semi-urban and rural. * To determine the inter-individual variation of systemic and intraintestinal oxidation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural. * To describe the inter-individual variation of systemic and intraintestinal inflammation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural. * To determine the inter and/or intra-individual variation of urinary tract infection and gastrointestinal parasite infestation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural. * To describe the inter and intra-individual variation of the hydration status in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural. * To explore intra-individual associations in variables of the same research component or different component variables.
Please note protocol is also available in Spanish.
Study Type
OBSERVATIONAL
Enrollment
91
Center for Studies of Sensory Impairment Aging and Metabolism
Guatemala, Departamento de Quetzaltenango, Guatemala
Weight
Growth, measured trough weight (kg)
Time frame: 7th week of observation
Hemoglobin
Hemoglobin (mg/dl) measured in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango, Guatemala.
Time frame: 8th week of observation
Urine Osmolality-1
Hydration Status= measured in 24-h urine through urine volume and osmolality in osmometer Model Löser 815. Results in mOsm/kg.
Time frame: 6th week of observation
Height
Growth, measured trough height (cm).
Time frame: 7th week of observation
Urine Osmolality-2
Hydration Status= measured in 24-h urine through urine volume and osmolality Osmometer Model Löser 815. Results in mOsm/kg.
Time frame: 7th week of observation
Urine Osmolality-3
Hydration Status= measured in 24-h urine through urine volume and osmolality in two different osmometers (Löser 815 and Osmomat 030). Results in mOsm/kg.
Time frame: 8th week of observation
Urine Urea
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: urea (mg/l)
Time frame: 8th week of oservation
Urine Uric Acid
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: uric acid (mg/l)
Time frame: 8th week of oservation
Urine Sodium
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: sodium (mg/l)
Time frame: 8th week of oservation
Urine Potassium
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: potassium (mg/l)
Time frame: 8th week of oservation
Urine Calcium
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: calcium (mg/l)
Time frame: 8th week of oservation
Urine Magnesium
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: magnesium (mg/l)
Time frame: 8th week of oservation
Urine Phosphorus
Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: inorganic phosphorus (mg/l)
Time frame: 8th week of oservation
Urine 15-f2t-Isoprostanes
Urine Oxidation biomarkers: 15-f2t-Isoprostanes (ng/mL) Measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada using ELISA assay kit (Oxford Biomedical Research, Inc., Catalog # EA84.102606, Michigan, USA)
Time frame: 8th week of observation
Urine 8-Hydroxydeoxyguanosine
Urine Oxidation biomarkers: 8-Hydroxydeoxyguanosine (ng/mL) Measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada using ELISA assay kits (JaICA, Nikken SEIL Co., Ltd, Catalog# IM-KOGHS 040914E, Shizouka, Japan).
Time frame: 8th week of observation
Catalase Activity
Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system: Catalase Activity (nmol/seg/g Hb)
Time frame: 8th week of observation
Superoxide Dismutase Activity
Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Superoxide Dismutase Activity (U/g Hb)
Time frame: 8th week of observation
Gluthathione Reductase
Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Gluthathione Reductase Activity (umol/min/g Hb)
Time frame: 8th week of observation
Gluthathione Peroxidase
Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Gluthathione Peroxidase Activity (uU/g Hb)
Time frame: 8th week of observation
Retinol
Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Retinol (mg/dl)
Time frame: 8th week of observation
Alpha-tocopherol
Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Alpha-tocopherol (mg/l)
Time frame: 8th week of observation
Beta-Carotenes
Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Beta-carotenes (mg/l)
Time frame: 8th week of observation
Coenzyme Q9
Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Coenzyme Q9 (mg/l)
Time frame: 8th week of observation
Coenzyme Q10
Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Coenzyme Q10 (mg/l)
Time frame: 8th week of observation
Plasma Interleukin-1B
Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-1B (pg/ml)
Time frame: 8th week of observation
Plasma Interleukin-6
Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-6 (pg/ml)
Time frame: 8th week of observation
Plasma Interleukin-8
Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-8 (pg/ml)
Time frame: 8th week of observation
Plasma Interleukin-10
Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-10 (pg/ml)
Time frame: 8th week of observation
Plasma Tumor Necrosis Factor-Alpha
Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma Tumor Necrosis Factor-Alpha (pg/ml)
Time frame: 8th week of observation
Saliva Interleukin-1B
Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-1B (pg/ml)
Time frame: 8th week of observation
Saliva Interleukin-6
Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-6 (pg/ml)
Time frame: 8th week of observation
Saliva Interleukin-8
Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-8 (pg/ml)
Time frame: 8th week of observation
Saliva Interleukin-10
Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system Saliva IL-10 (pg/ml)
Time frame: 8th week of observation
Saliva Tumor Necrosis Factor-Alpha
Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva Tumor Necrosis Factor-Alpha (pg/ml)
Time frame: 8th week of observation
Fecal Calprotectin
Fecal Calprotectin (mg/kg) measured using the ELISA assay kit from Calpro-Lab (Catalog # CAL0100).
Time frame: 8th week of observation
Intestinal Parasitosis
Intestinal Parasitosis, measured by Biochemistry Laboratory, La Democracia Hospital, Quetzaltenango, Guatemala. Parasite cysts presence in feces.
Time frame: 8th week of observation
Giardiasis Infection Presence-1
Analyses done by ELISA assay kit (ProSpecT Giardia EZ, catalog #R2458596), absorbance (O.D)
Time frame: 7th week of observation
Giardiasis Infection Presence-2
Analyses done by ELISA assay kit (ProSpecT Giardia EZ, catalog #R2458596), absorbance (O.D)
Time frame: 8th week of observation
White Urine Cell Count
White urine cell count (per field), to be determined in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango Guatemala.
Time frame: 8th week of observation
White Blood Cell Count
White blood cell count (cells/ul), to be determined in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango Guatemala.
Time frame: 8th week of observation
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