Does DNA Sperm Fragmentation Affects Embryo Morphokinetic?

IVI Madrid60 enrolled

Overview

The embryokinetics may be a new prognostic factor for choosing the human embryos with the highest implantation potential. In order to identify the factors that may affect the rate of embryo cleavage, there are many studies about morphokinetics and culture medium, oxygen tension, oocyte quality or type of medication used for ovarian stimulation. However there are no published data with respect to the influence of sperm DNA fragmentation in the embryo kinetic.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Blastocyst Time Pronuclear Appearance Pronuclear Fading Embryo Compaction Cleavage Times

Eligibility

Sex: ALLMin age: 36 YearsMax age: 38 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patients 36 to 38 years * Fresh sample semen * Minimum 6 oocytes * Semen sample with at least 5 million/ml and at least 30% of progressive motile sperm. Exclusion Criteria: * Patients younger than 36 or older than 38 years old * Frozen semen sample * Less tan 6 oocytes. * Semen samples with less than 5 million / ml or less than 30% of motile sperm.

Outcomes

Primary Outcomes

Time of pronuclear appearance in hours.

We want to study whether differences exist in the time of appearance of pronuclei in those embryos that derived from semen samples from the two study groups the minimum value of the difference to be detected; we said that about 3 hours apart.

Time frame: The mean time of appearance of pronuclei is 3.3951 hours in our patients. We are going to evaluate pronuclear appearence time in hours in our study groups