Effect of Prebiotic and PUFA on the Gut Microbiota and Metabolic Risk Markers

Inclusion Criteria: * BMI: 25-40 kg/m2 * Non-smoking * Indices of the Metabolic Syndrome defined by the International Diabetes Federation (IDF 2006): Central obesity (waist circumference; men: ≥94 cm and women: ≥80 cm) + any one of the following; raised triglyceride level (≥1.7 mmol/L), reduced HDL cholesterol (men: \<1.03 mmol/L, women: \<1.29 mmol/L), raised BP (systolic ≥130 mm Hg or diastolic ≥85 mm Hg) or raised fasting plasma glucose (≥5.6 mmol/L). Exclusion Criteria: * Use of antibiotics * Weight change \>3 kg 2 months prior to study start * Intensive physical training/ elite athlete * Medication for dyslipidemia, type 2 diabetes or elevated blood pressure * Dietary supplements with pro/prebiotics, dietary fibre or fish oil supplement * Lactation, pregnancy or planning of pregnancy during the study * Gastro intestinal and liver disorders * Chronic inflammatory disorders * Diagnosed psychiatric disorder including treatment required depression * Surgical treatment of obesity and abdominal surgery