Intervention studies support a protective effect of flavonoid-rich foods such as chocolate and tea on cardiovascular disease risk. In a previous study we found that pure epicatechin improves markers of vascular function and insulin resistance although the results were less pronounced than the majority of chocolate intervention studies. The food matrix of chocolate or other compounds present in chocolate could play a role in the bioavailability of flavonoids, resulting in these heightened improvements in vascular function. In this study we aim to compare the acute effects of pure epicatechin and high-flavonoid chocolate on markers of vascular function.
Twenty male participants between the ages of 40 and 80 will visit the university on three days, separated by a 2-week washout. During each visit the participants will, in random order, be allocated to one of the three interventions: 1. High flavan-3-ol chocolate (70g) plus placebo capsule; 2. White chocolate (70g) plus 100mg of epicatechin in capsule form; 3. White chocolate (70g) plus placebo capsule; Measurements of vascular function will be taken before, and 2 hours after consumption of the supplements. In order to determine flavonoid bioavailability, 24-hour urine samples will be collected and 7 blood samples will be taken over a period of 8 hours. The primary study parameter is the percentage change in Flow-Mediated Dilation (FMD) as a result of a high-flavonoid cocoa or epicatechin challenge. Additional study parameters include plasma flavonoid and nitric oxide concentrations as well as pulse wave analysis parameters.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
20
Participants will consume 100mg of epicatechin (capsule) + 70g white chocolate
Participants will consume 70g high flavan-3-ol cocoa (100mg epicatechin) + placebo capsule
Participants will consume 70g white chocolate + placebo capsules
Wageningen University
Wageningen, Netherlands
Change in Endothelium-dependent flow-mediated dilation as a result of high flavan-3-ol cocoa or pure epicatechin consumption.
Change in arterial diameter as a result of 5 minute arterial occlusion (200mmHg). Arterial diameter will be measured by means of ultrasonography.
Time frame: Baseline and 2 hours after consumption
Change in nitric oxide as a result of high flavan-3-ol cocoa or pure epicatechin consumption
Plasma analysis of nitric oxide
Time frame: Baseline and 1 hr, 2hr, 3hr, 4hr, 6hr and 8hr after consumption
Changes in arterial augmentation index as measured by Pulse Wave Analysis
Measured in triplicate using a SphygmoCor device at baseline and 2 hours after consumption
Time frame: Baseline and 2 hours after consumption
Bioavailability of plasma epicatechin metabolites
Plasma analysis of epicatechin metabolites will be measured up to 8 hours after consumption of high flavan-3-ol cocoa or pure epicatechin
Time frame: Baseline and 1hr, 2hr, 3hr, 4hr, 6hr and 8hr after consumption
Bioavailability of urine epicatechin metabolites
Urinary epicatechin metabolites will be measured in 24-hour urine samples
Time frame: 24 hour urine samples
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