The important role played by the GI tract microflora on the metabolic health of an individual are increasingly recognized. In this respect, the initial studies of Metchinkoff that suggested a role of fermented food (specifically yoghourts ) to modulate the gut microflora have evolved into the concept of "probiotics". Probiotics are living microorganisms that bring a benefit to the host when administered in sufficient quantity (WHO/FAO, 2001). Here the investigators will study, in normal young male volunteers, the potential to modulate post-prandial metabolic and inflammatory responses by the administration of a yoghourt containing the probiotic Lactobacillus rhamnosus (LGG).
This is a prospective, randomized, double-blind and cross-over study designed to evaluate the potential of a probiotic (Lactobacillus rhamnosus) to modulate the post prandial metabolic and inflammatory response of normale healthy individuals. After a wash out period during which volunteers will have to follow a diet devoid of all milk or milk-derived products, they will receive, in random order, either a daily dose of the probiotic, or a daily dose of acidified milk (control) for a total of three consecutive weeks. At the end of each of these period, they will be submitted to a day of investigation, during which their metabolic and inflammatory profile will be studied following the ingestion of a standard test meal. The potential for the probiotic to modulate the metabolic and inflammatory parameters will be evaluated. The test meal will provide 1004Kcal under the form of 45.4g proteins (17%), 69g total fatty acids (62%), and 52.6g glucids (21%) as previously published (Schwander et al, J Nut 2014).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
14
Probiotic yogurt (fermented milk with Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus- YoFlex FD-DVS YC-X11 Fr, with the addition of Lactobacillus rhamnosus GG (LGG)- Culture Collection, University of Göteborg, Sweden CCUG 34291).
Milk will consist in an isocaloric supplement (compared to probiotic yogurt) of unfermented milk
Clinical research center, Lausanne University hospital
Lausanne, Canton of Vaud, Switzerland
Metabolomic analyses
Kinetics of metabolomic changes in blood during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Glucose
Kinetics of glucose during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Insulin
Kinetics of insulin during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Glucagon like polypeptide-1
Kinetics of GLP-1 during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Gastric inhibitory polypeptide
Kinetics of GIP during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Total cholesterol
Kinetics of total cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
LDL-cholesterol
Kinetics of LDL-cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
HDL-cholesterol
Kinetics of HDL-cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Non esterified fatty acids
Kinetics of NEFAs during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Triglycerides
Kinetics of triglycerides during the 6 hours after meal ingestion, in presence of yogurt or placebo
Time frame: 6 hours
Interleukin-6
Kinetics of IL-6 will be measured in blood of volunteers after the administration of yogurt or placebo
Time frame: 6 hours
Chemokine CCK2
Kinetics of CCK2 will be measured in blood of volunteers after the administration of yogurt or placebo
Time frame: 6 hours
Chemokine CCK5
Kinetics of CCK5 will be measured in blood of volunteers after the administration of yogurt or placebo
Time frame: 6 hours
Tunour necrosis factor alpha
Kinetics of TNF-a will be measured in blood of volunteers after the administration of yogurt or placebo
Time frame: 6 hours
Lipo-poly-saccharide
Kinetics of LPS will be measured in blood of volunteers after the administration of yogurt or placebo
Time frame: 6 hours
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.