Evaluation of 18 F-FP-DTBZ Pancreatic PET Scanning as a Tool to Measure Beta Cell Mass

Inclusion Criteria: * Patients with type 1 diabetes may be enrolled if they meet all of the following criteria: 1. Are males or females between 18 and 70 years of age, inclusive 2. Have a diagnosis of type 1 diabetes mellitus as defined by the American Diabetes Association (ADA) criteria or by diagnosed as per their endocrinologist; duration \>5 years; Insulin dose requirements \<0.8 units/kg/day 3. HbA1c level between 5% and 8.5% 4. Have fasting C-Peptide \< 0.1 ng/ml 5. Have a body mass index (BMI) between 18 and 32 kg/m2 6. Able to tolerate PET imaging 7. In the judgment of the physician, are capable of fasting 4 to 6 hours prior to screening and Day 1 imaging procedures 8. Give informed consent Healthy volunteers may be enrolled if they meeting all of the following criteria: 1. Are males or females between 18 and 70 years of age, inclusive 2. Have no history of type 1 or type 2 diabetes in a first degree relative 3. Fasting blood glucose less than 100 mg/dL 4. HbA1c level less than 6% 5. Normal Mixed Meal Tolerance test at screening visit 6. BMI between 18 and 32 kg/m2 7. Able to tolerate PET imaging 8. In the judgment of the physician, are capable of fasting 4 to 6 hours prior to screening and Day 1 imaging procedures, and 9. Give informed consent Exclusion Criteria: * Potential participants must not have any of the following exclusion criteria: 1. Clinically significant renal dysfunction 2. Clinically significant liver dysfunction as determined by history, physical examination, and standard liver function testing at screening (aspartate aminotransferase (AST), alanine aminotransferase (ALT), Total/Direct Bilirubin, Alkaline Phosphatase) 3. Coagulopathy 4. Use medications known to affect dopaminergic function, including monoamine oxidase (MAO) inhibitors, tetrabenazine, or levodopa 5. Recent (within 3 months) or current treatment with drugs influencing beta cell function or insulin sensitivity (e.g. glucocorticoids, reserpine) medications known to affect dopaminergic function, including MAO inhibitors, tetrabenazine, or levodopa 6. Have polycystic ovarian syndrome 7. History of movement disorder such as Parkinson's Disease, Huntington's Disease 8. Clinically significant psychiatric disease or history of psychiatric illness such as depression, bipolar disease, anxiety or schizophrenia 9. Current use (within past year) of cocaine, methamphetamine, and/or ecstasy (3,4methylenedioxymethamphetamine (MDMA)) 10. Have a recent history of alcohol or substance abuse or dependence 11. Clinically significant cardiovascular disease or clinically significant abnormalities on screening electrocardiogram (ECG) (including but not limited to QT-interval time corrected \> 450 msec) 12. Clinically significant pulmonary, renal or hepatic impairment, or cancer 13. Have clinically significant infectious disease, including acquired immune deficiency syndrome (AIDS) or human immunodeficiency virus (HIV) infection or previous positive test for hepatitis B, hepatitis C, or HIV. 14. Are women of childbearing potential not refraining from sexual activity or not using adequate contraception. Women must not be pregnant (negative serum human chorionic gonadotropin (hCG) at the time of screen) or breastfeeding at screening, and must agree to take appropriate steps not to become pregnant during for 30 days following the clinical trial 15. Require medications with a narrow therapeutic window (e.g., warfarin), are receiving any investigational medications, or have participated in a trial with investigational medications within the last 30 days 16. Weigh more than the manufacturer recommended limit for the PET/computed tomography (CT) camera being used 17. Any prior participation in other research protocols within the past month that involved radiation, with the exception of plain radiography studies (i.e., chest x-rays); And 18. Have received a diagnostic or therapeutic radiopharmaceutical within the past week