Isolation of Vitamin D Binding Protein Isoforms From Human Volunteers

Universitaire Ziekenhuizen KU Leuven24 enrolled

Overview

The purpose of this study is to isolate genotype-specific DBP/Gc from homozygous human volunteers and use these purified proteins to cross-validate existing or novel assays for this serum protein and to evaluate the binding characteristics of these variants of DBP/Gc for different vitamin D metabolites.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Vitamin D Deficiency

Interventions

Blood drawOTHER

Sampling of 100-150 ml of blood

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Generally healthy adults (≥ 18 years), non pregnant, with known DBP/Gc genotype Exclusion Criteria: * Inability to provide written informed consent * No children or pregnant women * Persons with known abnormal serum proteins

Locations (1)

UZ Leuven

Leuven, Belgium

Outcomes

Primary Outcomes

Vitamin D binding protein binding characteristics (affinity constant)

In vitro characterization

Time frame: single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer

Secondary Outcomes

Correlation coefficient (r) of different DBP assays

In vitro characterization

Time frame: single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer

Data from ClinicalTrials.gov

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