The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.
Study Type
OBSERVATIONAL
Enrollment
200
University Hospital Duesseldorf
Düsseldorf, Germany
Blood examination
Following parameters will be examined: * Blood count (red cells, white cells, platelets, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), mean platelet volume (MPV) ) * Creatinin * Urea * Glucose * Glycated hemoglobin (HbA1c) * Cholesterol * Triglyceride * C-reactive Protein * Apolipoprotein
Time frame: baseline
Protein expression
Determination with western blot
Time frame: baseline
Asymmetric dimethylarginine (ADMA) and Cytokine
Determination with enzyme-linked immunosorbent assay (ELISA).
Time frame: baseline
Microparticles
Determination with ELISA and flow cytometry
Time frame: baseline
Immunocytochemistry
Time frame: baseline
Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN)
Determination with reductive chemiluminescence
Time frame: baseline
Nitric oxid and reactive oxygen species
Determination with fluorescence coloring in flow cytometry and fluorescence microscopy
Time frame: baseline
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