To compare changes in follicular ovarian reserve in women before and six months after laparoscopic operation: endometrioma stripping surgery. Also, to compare follicular ovarian reserve in women with endometrioma(s) with follicular ovarian reserve of healthy infertile women of the same age group.
In order to investigate the impact of laparoscopic stripping surgery on follicular ovarian reserve, blood samples will be obtained before the operation, from 2nd to 5th day of the menstrual cycle, to determine levels of endocrine markers of ovarian reserve (FSH, E2, AMH and Inhibin B). Cancer antigen 125 (CA -125) and IL-6 will be measured also. US markers (antral follicular count- AFC) will be obtained at the same time as endocrine markers. All laparoscopic stripping surgery will be performed during the early or middle follicular phases of menstrual cycle, under general anesthesia, by the same team of gynecological endoscopist. The cyst wall will be completely stripped off from the normal ovarian tissue by traction and opposite traction with two grasping forceps. Hemostasis will be achieved using bipolar forceps electrocoagulation. All endometrioma specimens that will be obtained from operation will be submitted for pathology examination. One and six months after the laparoscopic cystectomy, from 2nd to 5th day of menstrual cycle, we will determine endocrine markers of ovarian reserve and levels of CA 125 and IL-6. The control group will be consisted of healthy women with infertility problem. From the second to the fifth day of the menstrual cycle we will take blood samples to determinate endocrine markers of follicular ovarian reserve: AMH, FSH, E2, Inhibin B.
Study Type
OBSERVATIONAL
Enrollment
100
General Hospital of Subotica
Subotica, Vojvodina, Serbia
RECRUITINGCCSerbia, Clinic for Gynecology and Obstetrics, School of Medicine, University of Belgrade
Belgrade, Serbia
RECRUITINGDepartment of Laboratory Medicine ''Konzilijum''
Belgrade, Serbia
RECRUITINGChange of follicle-stimulating hormone (FSH)
as the endocrine marker of ovarian reserve
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of estradiol (E2) levels
as the endocrine marker of ovarian reserve
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of Anti-mullerian hormone (AMH) levels
as the endocrine marker of ovarian reserve
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of Inhibin B levels
as the endocrine marker of ovarian reserve
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of antral follicular count (AFC)
as the US marker of ovarian reserve
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of CA -125 levels
as the inflammatory marker
Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy
Change of interleukin-6 (IL-6) levels
as the inflammatory marker
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Time frame: 2nd to 5th day of the cycle in which laparoscopy is performed and 2nd to 5th day of the cycle in 6th month after laparoscopy