The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
TRIPLE
Enrollment
20
Intake of 200 ml of pomegranate juice or pomegranate juice dilution during 21 days after exercise.
Protein carbonyls levels changes in plasma
Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein
Time frame: day 0 and 22
Malondialdehyde levels changes in plasma
Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein
Time frame: day 0 and 22
Blood biochemical parameters changes in plasma
Blood biochemical parameters is a composite outcome measure consisting in circulating plasma glucose (mg·dL-1), HDL-cholesterol (mg·dL-1), triglycerides (mg·dL-1), ferritin (ng·mL-1), lactate (mg·dL-1), sodium (mEq·L-1), potassium (mEq·L-1), creatine kinase (U·L-1), aspartate aminotransferase (U·L-1) and alanine aminotransferase (U·L-1) were analyzed through biochemistry methods.
Time frame: day 0 and 22
C-reactive protein levels changes in plasma
Determination by immunoassay of C-reactive protein (ng·mL-1) levels in plasma.
Time frame: day 0 and 22
Soluble E-selectin levels changes in plasma
Determination by immunoassay of soluble E-selectin (ng·mL-1) levels in plasma.
Time frame: day 0 and 22
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