This prospective study with sibling oocytes will evaluate the efficiency of two commercial single-step media, namely Sage 1-step and Continuous Single Culture Medium (CSCM), in terms of embryo utilization rates, blastocyst formation rates, embryo quality and pregnancy rates. Embryos will be cultured continuously for up to 6 days under identical conditions.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
QUADRUPLE
Enrollment
43
Half of each patient's oocytes will be randomly allocated to be cultured either in Sage 1-step medium (Origio) or in CSC medium (Irvine Scientific) for up to six days following oocyte retrieval.
Eugonia Assisted Reproduction Unit
Athens, Attica, Greece
Utilization rates
Utilization = embryos selected for transfer and cryopreservation
Time frame: 6 days post oocyte retrieval
Blastocyst formation
Number of blastocysts on day 5 post oocyte retrieval per fertilized oocytes
Time frame: 5 days post oocyte retrieval
Embryo quality on day 3
Time frame: 3 days post oocyte retrieval
blastocyst quality on day 5
Time frame: 5 days post oocyte retrieval
positive pregnancy test
Time frame: 15 days post oocyte retrieval
clinical pregnancy
presence of gestational sac with positive fetal heartbeat
Time frame: 7 weeks of gestation
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