The main objective of the trial is to measure the thermogenic effect of two different doses of whey protein microgels. It will be a randomized crossover double-blind design conducted in 20 subjects.
Primary objective and outcome: The primary objective of the trial is to measure the thermogenic effect of two different doses of whey protein microgels (WPM). Secondary objectives and outcomes: Explore the mechanism of action of WPM on thermogenesis including: Protein turnover in response to the dose and the source of protein (WPM versus casein) Insulinemic and glycemic response to the dose and source of protein (WPM versus casein) Design: The proposed study is a randomized double-blind, placebo-controlled, single center, crossover design. At the start of the study, subjects will be randomly assigned to 4 different groups in a crossover design, where they will be asked to consume a beverage containing Product 1: 0g of protein (maltodextrin control) Product 2: 30g of WPM Product 3: 50g of WPM Product 4: 50g of micellar casein (MC) Number of subjects: The number of subjects will be 15. Sufficient subjects should be recruited to provide a 30% drop-out rate. Therefore 20 subjects will be needed including drop-outs.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
TRIPLE
Enrollment
17
85.0 % maltodextrin,15.0% fat, 0% WPM
43.7% maltodextrin, 15.2% fat, 41.1% (30 g) WPM
16.3% maltodextrin, 15.2% fat, 68.5% (50 g) WPM
thermic effect of food
gas exchanges through indirect calorimetry
Time frame: 7 hours from baseline measurements
Whole body protein turnover
protein synthesis and breakdown
Time frame: 7 hours from baseline
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16.7% maltodextrin, 15.0% fat, 68.2% (50 g) MC