Excretion of Acetylamantadine (AA) by Lung Cancer Patients During a Chemotherapy Regimen

Overview

This study is designed to determine if changes in acetyl amantadine (AA) metabolism with systemic chemotherapy - reflective of SSAT1 activity - are predictive of response to systemic therapy in patients with lung cancer. Ten patients with adenocarcinoma and 10 with small-cell cancer who are at American Joint Committee on Cancer (AJCC) stages 3 or 4 at the time of diagnosis will participate. AA metabolites will be examined by ELISA prior to initiation of systemic chemotherapy and after the second cycle of therapy. Changes in the pattern of metabolites will be correlated with convention clinical and radiographic response criteria.

Evaluation of response to systemic therapy is dependant on clinical and radiographic changes assessed prior to and after several cycles of systemic therapy. The response rate for lung cancer to systemic therapy ranges from 30 - 85%, depending on histology and other clinical factors, but difficult to predict in individual patients. Spermine-spermidine acetyl transferase (SSAT1) activity is up-regulated in many cancers and may be a marker of response to therapy. Metabolism of acetyl amantadine (AA) is reflective of SSAT1 activity. In order to determine if change in SSAT1 activity is predictive of response to systemic chemotherapy, we propose the following pilot clinical trial. A total of 20 volunteer patients - 10 patients with adenocarcinoma of the lung and 10 patients with small-cell histology will be included. Eligibility includes patients with adenocarcinoma and small-cell lung cancer who are at AJCC stage III or IV at diagnosis. Volunteers will be informed that this study is examining the possibility that we can detect a therapeutic response by a change in their excretion of AA before it can be detected by conventional patient assessment methods. The chemotherapy regimen will be selected by the treating physician and will not be influenced by participation in this clinical trial. The usual treatment regimen for the entities described above is a platinum-based doublet, often cis-platin with etoposide or gemcitabine administered for 4 to 6 cycles. The therapeutic response rates with these drug regimens is about 40% for adenocarcinoma and 70% for small-cell carcinoma. Clinical response is usually assessed between the second and third cycles of chemotherapy. Biological Sampling Details Patient volunteers will be asked to fast overnight prior to the day of their first scheduled chemotherapy and to ingest an oral dose of 200 mg of amantadine hydrochloride (HCl) in the morning within an hour of arriving in clinic. The following biological specimens will be collected: blood (2 x 10 ml) at 2 and 4 hours after amantadine ingestion, saliva (2 x 5ml) immediately after the blood samples, and a total urine specimen between the time of the 2 blood samples. Patient will be instructed to empty the bladder immediately before the first blood sample and then collect the total urine produced until the end of the second blood sample (4 hours). Subsequently, the patient will be allowed lunch (if they so choose) prior to their scheduled chemotherapy. The sampling protocol will be repeated 3 weeks later with the second cycle of chemotherapy. Data to be collected: tumour type (histology), stage of cancer, age, sex, weight, height, smoking history, concurrent medications, systemic chemotherapy treatment prescribed and history of recent alcohol intake. Data will be used for correlation and to determine the relationship of plasma, salivary or urinary AA to the particular cancer diagnosis and response to treatment. Primary Endpoints: Documentation of acetylamantadine metabolite excretion/secretion. Response to therapy (complete response, partial response, stable disease, progressive disease). Time to disease recurrence or progression.