Study of the Safety and Immunogenicity of Pfs230D1M-EPA/Alhydrogel and Pfs25M-EPA/Alhydrogel , a Transmission Blocking Vaccine Against Plasmodium Falciparum Malaria, in Adults in the U.S. and Mali

* INCLUSION CRITERIA: (US \& Mali) All of the following criteria must be fulfilled for a volunteer to participate in this trial: 1. Age greater than or equal to18 and less than or equal to 50 years. 2. Available for the duration of the trial. 3. Able to provide proof of identity to the satisfaction of the study clinician completing the enrollment process. 4. In good general health and without clinically significant medical history. 5. Females of childbearing potential must be willing to use reliable contraception (as defined below) from 21 days prior to Study Day 0 to 3 months after the last vaccination. * Reliable methods of birth control include one of the following: confirmed pharmacologic contraceptives (parenteral) delivery; intrauterine or implantable device. * Reliable methods of birth control include concurrent use of a pharmacologic and a barrier method, i.e. two of the following: confirmed pharmacologic contraceptives (oral, transdermal) delivery or vaginal ring AND condoms with spermicide or diaphragm with spermicide. * Abstinence of potentially reproductive sexual activity. * Non-childbearing women will also be required to report date of last menstrual period, history of surgical sterility (i.e. tubal ligation, hysterectomy) or premature ovarian insufficiency (POI), and will have a baseline urine or serum pregnancy test performed. 6. Willingness to have blood samples stored for future research. 7. Willingness to undergo direct skin feeds (Mali only). 8. Known resident of Bancoumana or surrounding area (Mali only). EXCLUSION CRITERIA: (US \& Mali) A subject will be excluded from participating in this trial if any one of the following criteria is fulfilled: 1. Pregnancy as determined by a positive urine or serum human choriogonadotropin ( \<=- hCG) test (if female). NOTE: Pregnancy is also a criteria for discontinuation of any further dosing or nonsafety related interventions for that subject. 2. Currently breast-feeding (if female). 3. Behavioral, cognitive, or psychiatric disease that in the opinion of the investigator affects the ability of the participant to understand and comply with the study protocol. 4. Hemoglobin, WBC, absolute neutrophils, and platelets outside the local laboratorydefined limits of normal (subjects may be included at the investigator s discretion for not clinically significant values outside of normal range). 5. Alanine transaminase (ALT) or creatinine (Cr) level above the local laboratory-defined upper limit of normal (subjects may be included at the investigator s discretion for not clinically significant values outside of normal range). 6. Infected with human immunodeficiency virus (HIV), hepatitis C virus (HCV), or hepatitis B (HBV). 7. Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, endocrine, rheumatologic, autoimmune, hematological, oncologic, or renal disease by history, physical examination, and/or laboratory studies including urinalysis. 8. History of receiving any investigational product within the past 30 days. 9. Participation or planned participation in a clinical trial with an investigational product prior to completion of the follow up visit 28 days following last vaccination OR planned participation in an investigational vaccine study until the last required protocol visit 10. Subject has had medical, occupational, or family problems as a result of alcohol or illicit drug use during the past 12 months. 11. History of a severe allergic reaction or anaphylaxis. 12. Severe asthma, defined as asthma that is unstable or required emergent care, urgent care, hospitalization, or intubation during the past 2 years, or that has required the use of oral or parenteral corticosteroids at any time during the past 2 years. 13. Pre-existing autoimmune or antibody-mediated diseases including but not limited to: systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, Sj(SqrRoot)(Delta)gren s syndrome, or autoimmune thrombocytopenia. 14. Known immunodeficiency syndrome. 15. Known asplenia or functional asplenia. 16. Use of chronic (greater than or equal to 14 days) oral or intravenous corticosteroids (excluding topical or nasal) at immunosuppressive doses (i.e., prednisone \>10 mg/day) or immunosuppressive drugs within 30 days of Study Day 0. 17. Prior to Study Day 0 and every subsequent vaccination day, receipt of a live vaccine within the past 4 weeks or a killed vaccine within the past 2 weeks. 18. Receipt of immunoglobulins and/or blood products within the past 6 months. 19. Previous receipt of an investigational malaria vaccine in the last 5 years. 20. Other condition that in the opinion of the investigator would jeopardize the safety or rights of a participant participating in the trial, interfere with the evaluation of the study objectives, or would render the subject unable to comply with the protocol. 21. Prior malaria infection by history in the last 10 years (US only). 22. Prior travel to a malaria transmission area in the last 5 years or planned travel during the course of the study (US only). 23. History of severe reaction to mosquito bites (Mali only). 24. History of allergy to any component of the comparator vaccine (e.g. neomycin) (Mali only). INCLUSION AND EXCLUSION FOR LARGE VOLUME BLOOD DRAW: \- Individuals previously enrolled in Arms A2, B2, or C2 may be asked to return to the clinic for a single additional visit. This visit would be to collect a single large volume blood sample for additional serological analysis and characterization of B cell receptor usage. INCLUSION CRITERIA (LARGE VOLUME BLOOD DRAW): -The following criteria must be fulfilled for a volunteer to participate in the large volume blood draw addendum: \-- Previously enrolled in 15-I-0044 in Arms A2, B2, or C2 EXCLUSION CRITERIA (LARGE VOLUME BLOOD DRAW): \- A subject will be excluded from participating in the large volume blood draw addendum if any one of the following criteria is fulfilled: * Pregnancy as determined by self report (if female). * Within the past 30 days has received a research drug or vaccine or been enrolled in another research drug or vaccine trial. * Condition or on a medication that in the opinion of the investigator would jeopardize the safety or rights of a participant participating in the trial, interfere with the evaluation of the study objectives, or would render the subject unable to comply with the protocol.