HO-1 and Muscle Mitochondrial Dysfunction in Sepsis

Changhai Hospital50 enrolled

Overview

The current project was designed to examine dynamic changes in muscle wasting during sepsis. Researchers will focus the mitochondrial dysfunction of muscle cells and investigate the role of HO-1 in it. Researchers interested in identifying factors involved in the pathology of muscle wasting during sepsis.

Rapid muscle atrophy or muscle wasting occured to septic patients.Sepsis induced muscle wasting has become a severe, frequent, and persistent complication among critically ill patients. Multiple factors including ubiquitin-proteasome system, proinflammatory cytokines and oxidative stress contribute muscle proteolysis. It is known that heme oxygenase-1 (HO-1) possesses a variety of functions like anti-inflammatory, antioxidative and anti-apoptosis effects. In order to investigate the mechanisms under the condition, researchers are going to examine the role of HO-1 in muscle atrophy during sepsis.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Sepsis Atrophy

Interventions

therapy according to SSC guidelines and muscle biopsyPROCEDURE

Septic patients will be treated according to the international guidelines for management of severe sepsis and septic shock 2012. Skeletal muscle biopsies, blotted free from blood, are frozen within 30 seconds in liquid nitrogen, and stored in liquid nitrogen until processing.150-300 mg muscle (wet weight) was routinely obtained , which is more than sufficient for the analyses proposed, with some muscle remaining for any additional validation assays that may arise during the course of the research. Muscle will be processed either for isolation of mRNA/microRNA or protein

standard surgery for femoral fracture and muscle biopsyPROCEDURE

Patients with femoral fracture will under standard surgery for fracture fixation. Percutaneous needle muscle biopsies will be performed. Skeletal muscle biopsies, blotted free from blood, are frozen within 30 seconds in liquid nitrogen, and stored in liquid nitrogen until processing.150-300 mg muscle (wet weight) was routinely obtained , which is more than sufficient for the analyses proposed, with some muscle remaining for any additional validation assays that may arise during the course of the research. Muscle will be processed either for isolation of mRNA/microRNA or protein

Eligibility

Sex: ALLMin age: 18 YearsMax age: 90 Years

Medical Language ↔ Plain English

Inclusion Criteria: * sepsis, severe sepsis, septic shock * clinical reason for fixation surgery of femoral fractures Exclusion Criteria: * no informed consent * medical history of myopathy * unintended weight loss before surgery or ICU admission * chronic use of corticosteroids

Locations (1)

Anesthesiology and Intensive Care, Changhai Hospital

Shanghai, Shanghai Municipality, China

Outcomes

Primary Outcomes

HO-1 mRNA and protein expression, mitochondria function of musculus vastus lateralis

Time frame: 3 days after diagnosis

Secondary Outcomes

length of ICU stay

Time frame: 60 days

length of mechanical ventilation

Time frame: 60 days

muscle myosin content

Time frame: 3 days after diagnosis

Data from ClinicalTrials.gov

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