According to many recent research, mesenchymal stem cells (MSC) isolated from synovial membrane and infrapatellar fat pad have higher ex vivo proliferation capacity, multipotency and ability to undergo chondrogenesis. Based on this concept, the investigators plan to get two removed tissues (synovial membrane and infrapatellar fat pad) from patients who have an operation of total knee replacement. The investigators are going to isolate and expand MSC from these two tissues respectively, and then compare the chondrogenesis ability for clinical application. In the other hand, the investigators plan to build techniques and procedures which comply with "Good Tissue Practices (GTP)"
Study Type
OBSERVATIONAL
Enrollment
6
mesenchymal stem cell isolation
Far Eastern Memorial Hospital
Pan-Ciao, Taipei, Taiwan
RECRUITINGCell viability ratio of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
Time frame: 6 months
Doubling time of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
Time frame: 6 months
Percentage expression of cluster differentiation 90,105, 34, 45 surface markers of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
Time frame: 6 months
Alcian blue staining of synovial membrane MSC and infrapatellar fat pad MSC
Time frame: 6 months
Tyep II collagen and aggrecan gene expression of synovial membrane MSC and infrapatellar fat pad MSC
Time frame: 6 months
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