Understanding the Relationship Between Inflammation and Insulin Resistance in the Adipose Tissue

Mayo Clinic28 enrolled

Overview

This study is being done to better understand the relationship between inflammation and insulin resistance in your Adipose (fat) Tissue .

This study is being done to determine whether eosinophils (a type of white blood cell) play an important role in human fat metabolism and inflammation. This study is being done to determine if fish oil supplement could play a role in reducing inflammation and improving insulin sensitivity in your fat tissue.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

OTHER

Masking

NONE

Enrollment

Conditions

Obesity Insulin Resistance

Interventions

Fat biopsy during scheduled abdominal surgeryPROCEDURE

Physical exam including blood work, an EKG and bioimpedance testing. An oral glucose tolerance test. Insulin infusion. Fat tissue biopsy during a scheduled abdominal surgery.

Fat AspirationPROCEDURE

Physical exam including blood work, an EKG and bioimpedance testing. An oral glucose tolerance test. Insulin infusion. Subcutaneous fat tissue biopsy.

Fish Oil SupplementDIETARY_SUPPLEMENT

Fish Oil supplementation for three months.

Eligibility

Sex: ALLMin age: 30 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Lean, healthy person with a body mass index (BMI) of 25 or less, or you are obese, BMI between 30 and 50, and non-diabetic. Lean healthy controls will not be on any medication and will be undergoing elective abdominal surgery excluding those involving acute inflammation (i.e. acute cholecystitis, bowel perforation, or diverticulitis). Obese, non-diabetic subjects will have a BMI between 30 and 50 and be taking no medications affecting glucose metabolism or lipid metabolism. These subjects will be identified from a pool of patients undergoing an elective abdominal surgical procedure such as inguinal hernia repair, laparoscopic Nissen fundoplication, planned cholecystectomy, but also bariatric surgery. Volunteers will not be taking corticosteroid therapy or have a history of asthma, COPD or atopic syndrome. All subjects will undergo a medical history intake and a physical examination followed by an OGTT to confirm normal glucose.

Locations (1)

Mayo Clinic in Arizona

Scottsdale, Arizona, United States

Outcomes

Primary Outcomes

Eosinophil content in adipose tissue

Eosinophils number will be measured by flow cytometry in subcutaneous and visceral fat of lean vs obese, non-diabetic volunteers

Time frame: baseline

Secondary Outcomes

Relationship between eosinophils content and body mass index (BMI)

Measure as a linear regression between BMI (kg/m2) and eosinophils number in the fat

Time frame: baseline

Data from ClinicalTrials.gov

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