Bioavailability of Carotenoids and Flavonoids From Fresh Oranges and Orange Juice.

University of Hohenheim12 enrolled

Overview

The study was designed as a randomized 2-way cross-over study to assess the bioavailability of carotenoids and flavonoids from sweet oranges and orange juice. Volunteers followed a wash-out period for 2 weeks prior to eating either 400 g of fresh oranges or 719 g of orange juice. The amount of test food was adjusted to deliver an equal amount of β-cryptoxanthin (740 µg). After a consecutive wash-out period of 2 weeks, the procedure was repeated and the volunteers ingested the complementary test food. Blood samples were collected at 8 time points up to 9.5 h after test meal consumption. Additionally, 24 h-urine was collected to evaluate flavonoid recovery.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Healthy

Interventions

Eligibility

Sex: ALLMin age: 23 YearsMax age: 30 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy adult men and women * BMI: 17-60 * Age: 18-65 Exclusion Criteria: * Women who are lactating or pregnant or plan to be pregnant * Individuals using tobacco products * Individuals with metabolic or malabsorption disorders * Individuals with a history of cancer * Individuals with a history of liver insufficiency * Individuals with a history of chronic and lipid metabolism-related diseases * Individuals with an allergy to oranges * Obese individuals (BMI \> 30)

Outcomes

Primary Outcomes

Mean AUC (nmol/L*9.5 h) of beta-cryptoxanthin concentration in blood plasma chylomicrons after ingestion of fresh oranges and orange juice, respectively.

Time frame: 0 - 9.5 h after each intervention

Secondary Outcomes

Mean AUC of hesperidin- and narirutin-metabolite concentration in blood plasma after ingestion of fresh oranges and orange juice, respectively.