ACE Stapler Sub Study

Inclusion Criteria: Criteria for inclusion in main ACE stapling study: * Subject must be able to understand and be willing to sign an informed consent document. * Subject must be willing and able to participate in all aspects of the study and agree to comply with all study requirements for the duration of the study. This includes availability of reliable transportation and sufficient time to attend all follow-up visits. * Subject has a BMI of 40 - 45 or 30 to 39.9 plus one or more co-morbid diseases expected to improve with weight loss, including but not limited to hypertension, dyslipidemia, obstructive sleep apnea, or diabetes mellitus. * Subject must be fully ambulatory, without chronic reliance on walking aids such as crutches, walkers or a wheelchair. * Subject must be of sufficient and stable medical health, as evaluated by the Principal Investigator. * Subject must have a primary care physician that will manage the subject for any co-morbid conditions throughout the study. * Subject must have failed standard obesity therapy of diet, exercise, behaviour modification, and pharmacologic agents either alone or in combination, as assessed by an interview with a member of the study team at baseline. * Subject agrees to refrain from any type of reconstructive surgery that may affect body weight such as mammoplasty or abdominal lipoplasty or liposuction, during the trial. Inclusion criteria sub study • Patient must be included in the main study Exclusion Criteria: Main study * Subject has history of/or signs and/or symptoms of gastro-duodenal ulcer disease. * Subject has poorly controlled diabetes as indicated by the lack of stable diabetes medications and doses over the last month, or has a history of diabetes for greater than 10 years. * Subject has had significant weight loss in the last 3 months, or between baseline and the study procedure. * Subject has a history or is diagnosed with eating disorders. * Subject has history of peptic ulcer and tests positive for H. pylori, unless treated before the procedure. * Subject has symptomatic congestive heart failure, cardiac arrhythmia or unstable coronary artery disease. * Subject has pre-existing respiratory disease such as chronic obstructive pulmonary disease (COPD), pneumonia or cancer. * Subject has significant esophageal disease including Zenker's diverticulum, grade 3-4 reflux esophagitis, stricture, Barrett's esophagus, esophageal cancer, esophageal diverticulum, dysphagia, achalasia, or symptoms of dysmotility. * Subject is observed during EGD to have heavily scarred, malignant or poor quality/friable tissue in areas of the stomach where plications are to be placed. * Subject has renal and/or hepatic insufficiency. * Subject has thyroid disease which is not controlled with medication. * Subject has a history of intestinal strictures or adhesions. * Subject has systemic infection in the body at the time of the plication procedure. * Female subject who is pregnant (i.e., has a positive urine or blood pregnancy test prior to the procedure), is suspected to be pregnant, is lactating or is of childbearing potential but refuses to use adequate contraception during the study. * Female subject who started birth control pills less than 3 months before enrollment, or who plans to start taking birth control pills during the study. * Subject has had previous bariatric, gastric or esophageal surgery; intestinal obstruction; portal gastropathy; gastrointestinal tumors; esophageal or gastric varices, or gastroparesis. * Subject has severe coagulopathy (prothrombin time \> 3 seconds over control or platelet count \< 100,000) or is presently taking heparin, coumadin, warfarin, or other anticoagulants or other medications which impede coagulation or platelet aggregation. * Subject has chronic/acute upper GI bleeding conditions. * Subjects who are unable to discontinue use of aspirin and/or non-steroidal anti-inflammatory agents (NSAIDs) at least 14 days prior to a plication procedure and continuing for 14 days post-procedure. * Subjects undergoing chronic steroid therapy. * Subjects undergoing immunosuppressive therapy. * Subjects who cannot discontinue either prescription or over the counter weight loss medications for at least 30 days prior to the procedure as well as during the trial period. * Subjects who have started medications within the last 3 months that are known to cause weight gain. * Subjects who have cardiac pacemakers or other electronic implantable devices. * Subjects who have hiatal hernias greater than 2 cm. * Subjects who have current or potential neck masses that in the opinion of the investigator, may interfere with study-related procedures, or has a Mallampati (intubation) score greater than 3. * Subjects who have poorly controlled psychiatric disease including but not limited to manic-depressive disorder, schizophrenia, borderline personality disorder, depression or suicidal tendencies. * Subject has Crohn's disease or Ulcerative Colitis. * Subject currently uses or has a history of illicit drug(s) or abuses alcohol (defined as regular or daily consumption of more than 4 alcoholic drinks per day). * Subject has participated in a clinical study with an investigational new drug, biological, or therapeutic device within ≤ 28 days prior to enrollment in this study, and does not agree to abstain from participation in other clinical trials of any kind during this study. Exclusion criteria sub study * Presence of contra-indications for MRI and f-MRI, as mentioned in Attachment 1 and 2. * Hypersensibility to contrast (Dotarem) * Claustrophobia * Pregnancy