This study will test the influence of lactulose of the human gut microbiota. Healthy volunteers will ingest 50g of lactulose and donate stool samples 1 day before as well as 1-2 days and 14 days after the test. Using sequencing and metabolomics techniques the investigators will identify changes in microbiota composition upon lactulose exposure.
This is a clinical study for investigating the influence of lactulose on the composition and metabolic activities of the intestinal microbiota. We will recruit 40 healthy volunteers. Subjects will be randomized and receive either 50g lactose (intervention group) or 50g sucrose (control group). Subjects will donate fecal samples 24h before and after (1-2d, 14d) lactulose ingestion. After ingestion H2-in expiratory air and symptoms will be followed for 3 hours. Frozen samples will be analyzed for their microbiota composition and key metabolites.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
32
Subjects will ingest 50g of lactulose once
Subjects will ingest 50g of sucrose once
Division of Gastroenterology, University Hospital Zurich
Zurich, Switzerland
Influence of lactulose intake on gut microbiota composition (microbiota sequencing, analysis of E. coli)
microbiota sequencing, analysis of E. coli
Time frame: 1-2 days after ingestion of lactulose
Influence of lactulose intake on metabolic profiles within the fecal ecoystem (metabolomics analysis)
metabolomics analysis
Time frame: 1-2 days after ingestion of lactulose
Correlation of gut microbiota composition and H2-peak (H2-measurements)
H2-measurements
Time frame: within 3 hours after lactulose ingestion
Correlation of gut microbiota composition and symptoms (standardized record of symptoms)
standardized record of symptoms
Time frame: within 3 hours after lactulose ingestion
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