Test and evaluate a new diagnostic method (SRM-MS/MS: Selected Reaction Monitoring- Mass spectrometry) for Lyme Borreliosis on human skin biopsies. Patients included are those with the early skin manifestation (erythema migrans). This new proteomic method will be compared to the two existing method: culture of Borrelia and PCR detection of Borrelia (DNA detection of the bacteria).
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
84
Skin biopsies of patients with Lyme disease (erythema migrans) infected with Borrelia burgdorferi sensu lato will be analyzed by three diagnostic techniques : molecular technique (PCR), biological technique (culture of the bacteria) and proteomics technique (SRM/ MS-MS)
Centre Hospitalo-Universitaire Jean Minjoz
Besançon, France
Centre Hospitalier William Morey
Chalon-sur-Saône, France
Hôpital Pasteur
Colmar, France
Hôpital E. Muller
Mulhouse, France
Compare the sensitivity (percentage of positive tests) of the SRM / MS-MS to techniques of PCR and culture Borrelia
percentage of positive tests with all three techniques (SRM/MS-MS ; PCR ; culture)
Time frame: One year study
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Hôpital Robert Debré
Reims, France
Hôpitaux Universitaires de Strasbourg
Strasbourg, France
Hôpital Bel Air
Thionville, France