Chlorogenic acid (CGA) in coffee may lower the postprandial glucose response. CGA is destroyed by dark roasting. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light (LIR) or dark (DAR) roasted coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Plasma glukose and insulin, appetite, and plasma and urine metabolic profiles will be analysed. The primary aim is to investigate whether roasting affects the postprandial glucose area under the curve (AUC).
Epidemiologic evidence suggests that coffee consumption is associated with a lower risk of type 2 diabetes. Coffee contains caffeine and several other components that may modulate glucose regulation. The chlorogenic acids (CGA) in coffee have been indicated as constituents that may help to normalize the acute glucose response after a carbohydrate challenge. The aim of this study was to investigate whether two coffee beverages that differ in CGA content due to different roasting degrees will affect glucose regulation differently. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light roasted (LIR) or dark roasted (DAR) coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Blood samples were drawn at baseline, 30, 60 and 120 min. Differences in glucose and insulin responses and insulin sensitivity index (ISI) were analyzed. The CGA and caffeine contents in the coffees were analyzed using UPLC-MS/MS.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
11
Volunteers ingested 300 mL of coffee with a dark roast containing low levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
Volunteers ingested 300 mL of coffee with a light roast containing high levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
Volunteers ingested 300 mL of water containing no chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
Department of Nutrition, Exercise and Sports, University of Copenhagen
Frederiksberg, Denmark
Plasma glucose Area Under the Curve (Glucose AUC)
The area under the plasma glucose concentration curve was calculated from 0-120min after 75g glucose was ingested.
Time frame: 0-120 min
Plasma glucose concentrations
changes in the glucose concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.
Time frame: 30, 60 and120 min
Serum Insulin Area Under the Curve
Area under the curve (AUC) for serum insulin
Time frame: 0-120 min
Serum insulin concentrations
changes in the insulin concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.
Time frame: 30, 60 and120 min
ISI(0-120)
Matsuda's Insulin sensitivity index
Time frame: 0-120min
Metabolic profile in plasma
Metabolic profile of plasma measured in all samples collected before the meal and postprandially from 0-120 min as well as in samples collected up to 24 hrs later.
Time frame: 0-24 hours
Metabolic profile in urine
Metabolic profile of urine samples measured in all samples collected before the meal and postprandially from 0-120 min 120-240min as well as in samples collected up to 24 hrs later.
Time frame: 0-24 hours
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