Development and Validation of in Vitro Cell-based Bioassays for Nuclear Receptor Activation

Universitaire Ziekenhuizen KU Leuven73 enrolled

Overview

This is a prospective biobank set up to collect serum from patients with disorders associated with changes in androgens, estrogens or sex hormone-binding globulin (SHBG), before and/or after treatment.

In vitro cell-based bioassays may be a technique to measure serum androgen or estrogen bioactivity. In this study investigators are collecting serum (and if possible, urine) samples from different cohorts of patients, before and/or after treatment. The aim is to measure androgens, estrogens and SHBG as well as in vitro bioactivity with newly developed cell-based bioassays, and to compare different techniques to each other as well as to clinical endpoints available in the patient's Electronic Health Record.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Hypogonadism Estrogens Testosterone Androgens

Interventions

Serum (with or without urine) samplesOTHER

Eligibility

Sex: ALLHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Known or expected medical condition affecting androgens, estrogens or SHBG Exclusion Criteria: * Inability to provide written informed consent * Known or suspected bloodborne infectious diseases (e.g. HIV, viral hepatitis etc.) * Anemia (\<11 g/dL hemoglobin) or being a Jehovah's Witness