This study is designed to investigate whether protein supplementation can improve recovery of muscle function following a strenuous combination of both endurance and resistance exercise. It will specifically investigate the effect of protein supplementation on the recovery of strength, power and endurance exercise performance, along with measures of damage and inflammation of the muscle.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
SUPPORTIVE_CARE
Masking
DOUBLE
Enrollment
24
Whey protein hydrolysate (20 g)
Iso-energetic carbohydrate (20 g)
Negligible energy placebo (20 g)
GSK Investigational Site
Brentford, United Kingdom
Isometric Maximal Voluntary Contraction (MVC) Assessment
Maximum voluntary isometric contraction (MVIC) will be assessed at the knee during a leg extension movement using an isokinetic dynamometer. MVIC will be used to assess strength. Participants will perform the exercise using the dominant leg at a knee joint angle of 70º three times, with a contraction of 3 seconds (s) on each occasion (each contraction separated by 60 s rest). The peak force (measured in Newton) generated across the three repetitions will be recorded.
Time frame: 96 hours (h)
Counter Movement Jump (CMJ) Assessment
Power will be assessed using counter-movement jump. Participants will place their hands on their hips, descend rapidly to \~90º knee joint angle, and then jump as high as possible using a counter-movement. Three jumps will be performed (each jump separated by 60 s rest) and the peak vertical jump height (measured in centimeters) will be recorded.
Time frame: 96 h
16.1 Kilometer (km) Cycling Time Trial (TT) Assessment
The 16.1 km cycling TT assessment will be performed on a cycling ergometer. Participants will be required to complete a distance of 16.1 km in as short a time as possible, while being blinded to time elapsed.
Time frame: 96 h
Serum Creatine Kinase Analysis
Muscle damage will be assessed by analyzing serum creatine kinase (CK) using CK N-Acetyl-L Cysteine (NAC)-activated Enzyme Linked Immunosorbent Assay (ELISA).
Time frame: 96 h
Serum C-Reactive Protein Analysis
Muscle inflammation will be assessed by analyzing serum high sensitivity (hs) C-reactive protein (CRP) using hs-CRP ELISA.
Time frame: 96 h
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Perceived Muscle Soreness Assessment
Perceived muscle soreness will be measured on a 200 mm visual analogue scale (VAS) where 0 mm= no pain and 200 mm= unbearably painful. Participants will be required to hold a fixed squat position (90º knee joint angle) while perceived muscle soreness is rated.
Time frame: 96 h