Effect of AT-derived miRNA on the Biology and Insulin Sensitivity of Skeletal Muscle in Humans

Inclusion Criteria: * Able to communicate meaningfully with the investigator and legally competent to provide informed written consent * 18-65 years of age * BMI of 20-25 kg/m2 (lean subjects); 30-35 kg/m2 (obese subjects) * Stable body weight (\< 3 kg change in the last 8 weeks) * homeostatic model assessment (HOMA)-insulin resistance \<2.7 if lean; homeostatic model assessment (HOMA)-insulin resistance ≥2.7 if obese Exclusion Criteria: * Lactation or pregnancy, current and/or within last 6 months, per participant's report * Female subjects postmenopausal * Cardiovascular disease (unstable angina, myocardial infarction or coronary revascularization within 6 months) * Liver disease (AST or ALT(alanine aminotransferase)\>2.5 times the upper limit of normal) * Kidney disease (creatinine \>1.6 mg/dl) * Anemia (hemoglobin \<12 g/dl in men, \<11 g/dl in women) * Thyroid dysfunction (abnormal TSH) * HbA1c ≥6.5% * Uncontrolled hypertension (systolic BP\>160 mmHg, diastolic BP\>100 mmHg) * History of coagulopathies * History (within the last 5 years) or presence of malignancy, (skin cancers, with the exception of melanoma, may be acceptable) * Current or history of drug abuse or alcohol abuse (\>2 drinks/day) * Prior treatment (within last 3 months) with systemic glucocorticoids (\>2 weeks), beta-blockers, drugs for weight loss, niacin or fibrates * History of HIV, active Hepatitis B or C, or Tuberculosis (participant reported) * Smoke \> 5 cigarettes per day