The purpose of this study is to determine whether full-mouth disinfection is effective in the initial periodontal treatment of generalized aggressive periodontitis on clinical parameters, gingival crevicular fluid interleukin-1β (IL-1β) and interleukin-17 (IL-17) and periodontal pathogen levels compared with conventional initial periodontal treatment and full-mouth initial periodontal treatment. The investigators' hypothesis is to test whether full-mouth disinfection in the initial periodontal treatment of generalized aggressive periodontitis enhance the clinical, biochemical and microbiological parameters in comparison to conventional initial periodontal treatment and full-mouth initial periodontal treatment.
The present study aimed to compare the efficacy of conventional initial periodontal treatment (C-IPT), full-mouth disinfection IPT (FMD-IPT) and full-mouth IPT (FM-IPT) and the levels of gingival crevicular fluid interleukin-1β (IL-1β) and interleukin-17 (IL-17), Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, Parvimonas micra and Campylobacter rectus in patients with generalized aggressive periodontitis (GAgP) over 6-months period. Forty-two GAgP patients were randomly assigned into 3 groups. IPT was performed in a quadrant-wise manner at 1-week intervals in C-IPT, in 2 sessions within 24 hours in FM-IPT, and in 2 sessions with application of chlorhexidine to the intra-oral niches within 24 hours in FMD-IPT. FMD-IPT group also used daily 0,2% chlorhexidine for 3 weeks. At baseline, 3 and 6 months clinical parameters consisting of plaque index, gingival index, probing depth, bleeding on probing and clinical attachment level were recorded, gingival crevicular fluid and microbiological samples were collected. Gingival crevicular fluid levels of IL-1β and IL-17 were analyzed using ELISA. The quantitative real-time polymerase chain reaction method was used for the quantitative detection of periodontal pathogens.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
42
Initial periodontal treatment was performed in 2 sessions with application of chlorhexidine to the intra-oral niches within 24 hours (Klorhex® Gel 1% for 10 minutes, Klorhex® Spray 0,2% and Klorhex® rinse 0,2% for 3 weeks).
Initial periodontal treatment was performed in a quadrant-wise manner at 1-week intervals.
Initial periodontal treatment was performed in 2 sessions within 24 hours.
Probing Depth
Time frame: 6 months after initial periodontal treatment
Gingival Crevicular Fluid Interleukin-1β and Interleukin-17 Levels (pg)
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of A. Actinomycetemcomitans
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of Porphyromonas Gingivalis
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of Fusobacterium Nucleatum
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of Parvimonas Micra
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Bleeding on Probing
Possible score for BOP range from %0 (no sites with bleeding on probing) to %100 (all sites with bleeding on probing). Higher scores mean worse outcome.
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Plaque Index
Possible scores for Plaque Index range from 0 (no plaque) to 3 (visible plaque all around the tooth). Higher scores mean a worse outcome
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Gingival Index
Possible score for Gingival Index range from 0 (healthy gingiva) to 3 (severe gingivitis with bleeding). Higher scores mean worse outcome
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Clinical Attachment Level
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of Prevotella Intermedia
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
Level of Campylobacter Rectus
Time frame: Baseline, 3 and 6 months after initial periodontal treatment
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