The purpose of the present study is to visualize the inflammatory response and coagulation disorders during cardiac surgery in order to identify possible predictors for acute lung injury postoperatively.
Acute lung injury after cardiac surgery is a common complication. The pathophysiology is complex and still not fully elucidate. No specific biomarkers are identified and the diagnosis is solely based on impaired oxygenation. The purpose of the present study is to identify biomarkers of inflammation and coagulation in blood withdrawn simultaneous from the pulmonary artery and the left atrium during surgery and up to 20 hours after weaning from cardiopulmonary bypass.
Study Type
OBSERVATIONAL
Enrollment
50
No treatment
Acute lung injury measured as the fraction of partial pressure of oxygen divided by the fraction of inspired oxygen
Arterial blood gas analysis
Time frame: Evaluated 72 hours after weaning from cardiopulmonary bypass (CPB)
Interleukin-1beta
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interleukin-2
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interleukin-4
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interleukin-6
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interleukin-8
Measured in blood samples from radial artery, pulmonary artery and left atrium
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Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interleukin-10
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Tumor necroting factor-alpha
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Interferon-gamma
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Granulocyte-macrophage colony-stimulating factor
Measured in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB
Thrombin-antithrombin complexes
Measured in blood samples from pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB
Plasmin-antiplasmin complexes
Measured in blood samples from pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB
D-dimer
Measured in blood samples from pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB
Thrombin Fragment 1.2
Measured in blood samples from pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB
Metabolomics
Metabolomics using the NMR-technique in blood samples from radial artery, pulmonary artery and left atrium
Time frame: The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB