Acute Effects of Spices on Pathways Associated With Glucose Homeostasis and Inflammation on PBMCs of Healthy Subjects

Lund University12 enrolled

Overview

This study aims to investigate the effect of spices on gene expression of pathways associated with glucose homeostasis, oxidative stress and inflammation, in the postprandial phase in healthy human subjects.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Healthy

Interventions

Fat challenge breakfastOTHER

In this intervention, subjects are asked to consume 200 ml control drinks (non-energy flavored water). Following 10 min. beverage consumption, subjects will be given standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20

Fat challenge breakfast+spicesOTHER

In this intervention, subjects are asked to consume 200 ml spices drinks (non-energy flavored water + spices extract). Following 10 min. beverage consumption, subjects will be given standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20

Carbohydrate challenge breakfastOTHER

In this intervention, subjects are asked to consume 200 ml control drinks (non-energy flavored water). Following 10 min. beverage consumption, subjects will be given standardized high carbohydrate breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 60:20:20

Eligibility

Sex: ALLMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy males and females * BMI 20-28 kg/m2 * Must be able to accept spices * Agreed and signed informed consent Exclusion Criteria: * Below 18 years old * Uncomfortable speaking English and/or difficulties in understanding spoken English * Smoking or using snuss * Vegetarian or vegan * Stressed by venous blood sampling or previous experience of being difficult to be cannulated * Receiving any drug treatment that may influence the study outcomes * Pregnancy or breastfeeding

Locations (1)

Food for Health Laboratory - Lund University

Lund, Skåne County, Sweden

Outcomes

Primary Outcomes

Change from Baseline (0 hr) in untargeted PBMC gene expression profile at 2 hr and 4 hr

The venous blood samples will be taken for isolation of peripheral blood mononuclear cells. Extracted total RNA will then be used for running RNA-seq/whole-transcriptome shotgun sequencing (Illumina, San Diego, CA, USA)

Time frame: Prior to the initial intervention at 0 hr and after intervention at 2 hr and 4 hr.

Secondary Outcomes

Subjective appetite ratings using standard subjective 100 mm VAS

Appetite will be assessed using standard subjective 100 mm VAS (visual analogue scale) at time intervals throughout each visit

Time frame: Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min.

Metabolic biomarkers

The capillary blood samples will be taken for blood glucose analysis. The venous blood samples will be taken for further assessment of insulin, triacylglycerol, NEFA and inflammatory biomarkers (i.e. IL-6, IL-8, CRP)

Time frame: Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min.

Metabolite profile

Metabolite profile will be analyzed using Liquid Chromatography-Mass Spectrometry based methods

Time frame: Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min.

Data from ClinicalTrials.gov

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