Evaluation of the effect of conventional and piezo-surgery on the secretion of Heat Shock Protein (HSP70) and on the vial bone cells at the peripheral bone cut sides after the removal of impacted mandibular third molars on both sides of the jaw.
The study is about measuring the amount of HSP70 using Elisa kit for detecting HSP70 and mason trichrome for histological findings in which the study samples are patients who have bilateral impacted mandibular third molar to be extracted with bone removal. First we collect bone sample for mason trichrome with either method then we collect the second samples after removing most of the bones cover the impacted third molar for checking HSP70 levels. Then the bone samples are treated using a special protocol to eliminate the protein from bone samples for the Elisa analysis. The other bone samples are going to be studied under the microscope.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Enrollment
15
This intervention is dependent on use of piezosurgery to conduct the required bone cuts to remove third molars.
Department of Oral and Maxillofacial Surgery, University of Damascus Dental School
Damascus, Damscus, Syria
Levels of Heat Shock Protein 70
This will be measured using ELISA methodology
Time frame: Five minutes before third molar removal with the last removed layer of bone
Percentage of viable bone cells
Percentage of viable bone cells will be calculated based on the counts of viable bone cells observed under the microscope.
Time frame: This variable will be measured from the specimens taken within the first 15 minutes of bone cutting to remove third molars
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