Prognostic factors in Inflammatory Bowel Diseases (IBD) are currently mainly based on clinical factors (disease extension, perianal involvement, need for surgery, use of immunomodulators…). All of immunological markers (or serological) of IBD have a diagnostic role in indeterminate colitis (ulcerative colitis vs crohn's disease) but they never have been considered as predictors of IBD course in adults. Among the most used, anti-neutrophil cytoplasm antibodies (ANCA) and Anti-Saccaromyces cerevisiae antibodies (ASCA) allow the distinction between ulcerative colitis (ANCA+/ASCA-) and Crohn's disease (ANCA-/ASCA+), and their combined use has a sensitivity and a specificity of about 85%. However, 10 other antibodies have been identified and recently evaluated individually in IBD and especially in pediatric Crohn's disease: anti-ompC, anti-I2, anti-flagellins, anti-glycan (anti-laminaribioside carbohydrate antibodies (ALCA), anti-mannobioside carbohydrate antibodies (AMCA), anti-chitobioside carbohydrate antibody (ACCA), anti-chitin and anti-laminarin), anti-goblet cells and anti-C.albicans specific mannans antibodies. These complementary tests improve the reliability of the diagnosis. In a previous cross-sectional work on a cohort of 195 IBD patients, the investigator showed a prognostic role of some of anti-glycan Abs and especially a correlation with a pejorative form of the disease both in Crohn's disease than in Ulcerative Colitis (UC) and a prediction of corticodependency in IBD.
There is few data on the stability of these antibodies, most of the studies are cross-sectional. There are conflicting results among scarce longitudinal data. One study reported a negativation of anti-glycan antibodies in some cases but not of ASCA or ANCA. On the cohort of 195 patients included in the first study, the investigator would like to assess at 3 years the immunological profiles of these patients and thus to compare them. In case of modification of the serological status for some antibodies, the search for associated factors (clinical, biological or therapeutic) will be performed. In case of sero-negativation of anti-glycan antibodies, this could be linked with a decrease or a normalization of the increased intestinal permeability in IBD. Indeed, in this subgroup of patients, we will test this hypothesis by analyzing intestinal permeability in anti-glycan positive group on the 2 samples and in the group with a sero-negativation on the second sample.
Study Type
OBSERVATIONAL
Enrollment
80
blood specimen
CHU Saint-Etienne
Saint-Etienne, France
immunological status
Immunological status is defined by anti-glycan antibodies (ACCA, ALCA, AMCA, anti-chitin and anti-laminarin), ASCA and ANCA. Antibody will be positive if level is found higher than the threshold defined by the laboratory (technical threshold). An antibody will be defined as stable if its status remains positive during 3 years(above the detection limit given by the reference laboratory) or negative during 3 years (below the detection limit given by the reference laboratory). Conversely, the lack of stability during 3 years will be defined as the transition from a positive to a negative status or inversely.
Time frame: 3 years after first evaluation
clinical remission
Comparison of clinical remission between patients with a change in their immunological status and patients with stable status. A patient is in clinical remission if: 1. Crohn's disease activity index (CDAI) score is lower than 150 for Crohn's disease 2. Lichtiger score is \< 4 for Ulcerative Colitis
Time frame: 3 years after first evaluation
anti-Tumor Necrosis Factor (TNF) therapeutic response
Comparison of anti-TNF (Tumor Necrosis Factor) therapeutic response between patients with a change in their immunological status and patients with stable status. A patient is considered as anti-TNF responder if there is a clinical remission without therapeutic change in the medical history of the patient
Time frame: 3 years after first evaluation
Mucosal healing
Comparison of mucosal healing between patients with a change in their immunological status and patients with stable status. A patient will be considered in mucosal healing if: 1. C-reactive Protein (CRP) \< 5mg/L and fecal calprotectin \<250 µg/g 2. Fecal calprotectin \<150 µg/g in Ulcerative colitis
Time frame: 3 years after first evaluation
Intestinal permeability
Comparison of intestinal permeability between patients with a change in their immunological status and patients with stable status. 4\. Normalization of intestinal permeability will be defined if lipopolysaccharide (LPS) or anti-LPS Antibodies (IgG) level measured by LAL or ELISA is significantly reduced between the two samples. As there is no pre-defined threshold for LPS and anti-LPS Antibodies level in normalization of intestinal permeability, we will calculate a delta (level before - level at 3 years) for each patient and each parameter. We will analyze the average delta with the stability or not of immunological status.
Time frame: 3 years after first evaluation
surgical resection
Comparison of number of surgical resection between patients with a change in their immunological status and patients with stable status. 5\. Surgical resection may be a colonic or small bowel resection surgery for CD patients or colectomy for UC patients.
Time frame: 3 years after first evaluation
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