The purpose of this study is to establish the methodology of bronchial provocation tests with house dust mites in China, and to evaluate its safety and effects on upper and lower airways inflammation.
After screening,each participant undergo baseline lung function test,airway inflammation evaluation (blood and sputum eosinophils count,interleukin-13 (IL-13),FeNO)and airway responsiveness tests(methacholine bronchial provocation,nasal histamine provocation). On the second day,the participant undergo bronchial house dust mites challenge tests. On the third day,the participant undergo the lung function test,airway inflammation evaluation (blood and sputum eosinophils count,IL-13,FeNO)and airway responsiveness tests(methacholine bronchial provocation,nasal histamine provocation).
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
30
house dust mites(two-fold increasing concentration of house dust mite (HDM) extract, dilutions prepared starting from 1:8, 1:16, 1:32, 1:64, 1:128, 1: 256, 1:512, 1:1024,……)
Guangzhou institute of respiratory disease
Guangzhou, Guangdong, China
percentages of the participants that tested positive to house dust mites bronchial provocation
Time frame: 30 minutes after house dust mites bronchial provocation
Changes of the percentages of eosinophils in sputum, nasal lavage and fractional exhaled nitric oxide (FeNO) from baseline
Time frame: 30mins, 7 and 24 hours after bronchial challenge
Changes of provocative concentration induced a 20% decrease in forced expiratory volume at one second (FEV1) to methacholine before and after house dust mites bronchial provocation
Time frame: 24 hours after house dust mites bronchial challenge
percentages of the participants that tested positive to house dust mites bronchial provocation measured by impulse oscillometry
Time frame: 10 minutes after house dust mites bronchial provocation
Changes of provocative concentration induced a 60% increase in nasal airway resistance to histamine before and after house dust mites bronchial provocation
By Comparison with methacholine provocation test
Time frame: 24 hours after house dust mites bronchial challenge
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