Effects of E-smoke on Levels of Endothelial Progenitor Cells and Microparticles in Healthy Volunteers

Karolinska Institutet16 enrolled

Overview

We plan to investigate the acute effects of inhaling e-cigarette vapor on cell function measured by microvesicles and endothelial progenitor cells. Micro vesicles are released upon either activation or apoptosis from different cell types such as platelets, leucocytes and endothelial cell. Endothelial progenitor cells are a type of stem cells that circulate in the blood with the ability to differentiate to endothelial cells. Endothelial progenitor cells are inversely correlated to cardiovascular risk factors. As a secondary endpoint we plan to investigate exhaled nitric oxide - a common inflammation marker used in asthmatic patients - after inhalation of electronic cigarette vapor.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Healthy

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * healthy subjects * sporadic cigarette smoking (\<9 cigarettes per month) Exclusion Criteria: * Any form of cardiovascular disease * Any form of pulmonary disease like asthma or COPD * Any form of systemic or chronic disorder like rheumatologic or metabolic diseases. * Active allergy within 4 weeks of the study * Symptoms of infection or inflammation within 4 weeks of the study * Pregnancy

Outcomes

Primary Outcomes

Change in number of endothelial progenitor cells

Time frame: 24 hours

Change in number of micro vesicles

Time frame: 24 hours

Secondary Outcomes

Change in exhaled nitric oxide

Time frame: 24 hours

Effects of E-smoke on Levels of Endothelial Progenitor Cells and Microparticles in Healthy Volunteers

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes