The main objective of this study is to investigate the effect of a 4-weeks intake of berry peel powder on fasting and postprandial glucose metabolism and inflammatory markers. The berry peel product will be compared with a placebo in a single-blind, cross-over design. Furthermore, inflammation-related peripheral blood mononuclear cells (PBMCs) genes expression, appetite and cognitive performance will be included as a pilot study. Investigators hypothesize that long-term consumption of polyphenols and fibres of berry peel will exhibit both direct and indirect actions in healthy overweight subjects by improvement of glucose-associated markers as well as ameliorating inflammation. Moreover, above metabolic markers will correlated with improvements in inflammation-related PBMCs genes expression, subjective appetite and cognitive performance after long-term consumption of berry peel as a source of polyphenols.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
23
Food for Health Science Centre (Medicon Village) - Lund University
Lund, Skåne County, Sweden
Postprandial blood glucose response after acute intervention
The capillary blood will be collected for glucose analysis using a glucometer.
Time frame: -30, 0, 15, 30, 45, 60, 120, and 180 min after challenge meal.
Incremental area under curve (iAUC) blood glucose response after long-term intervention
The capillary blood will be collected for glucose analysis using a glucometer.
Time frame: 4 weeks
Postprandial serum insulin response after acute intervention
The capillary blood will be collected for insulin analysis using commercial ELISA kits
Time frame: -30, 0, 15, 30, 45, 60, 120, and 180 min.
Incremental area under curve (iAUC) serum insulin response after long-term intervention
The capillary blood will be collected for insulin analysis using commercial ELISA kits
Time frame: 4 weeks
Postprandial subjective appetite rating after acute intervention
Appetite will be assessed using standard subjective 100mm visual analogue scaled (VAS).
Time frame: -30, 0, 15, 30, 45, 60, 120, and 180 min.
Incremental area under curve (iAUC) subjective appetite rating after long-term intervention
Appetite will be assessed using standard subjective 100mm visual analogue scaled (VAS).
Time frame: 4 weeks
Postprandial C-reactive protein (CRP) after acute intervention
The venous blood will be collected for CRP analysis using ELISA commercial kits
Time frame: -30, 60, 120, and 180 min.
Incremental area under curve (iAUC) of C-reactive protein (CRP) after long-term intervention
The venous blood will be collected for inflammatory analysis using ELISA commercial kits
Time frame: 4 weeks
Postprandial adiponectin after acute intervention
The venous blood will be collected for adiponectin analysis using ELISA commercial kits
Time frame: -30, 0, 60, 120, and 180 min.
Postprandial adiponectin after long-term intervention with berry peel
The venous blood will be collected for adiponectin analysis using ELISA commercial kits
Time frame: 4 weeks
Peripheral blood mononuclear cells (PBMCs) inflammatory genes expression after acute intervention
The venous blood samples will be taken for isolation of peripheral blood mononuclear cells (PBMCs). Extracted total RNA will then be used for running quantitative-PCR.
Time frame: 180 min after challenge meal.
Peripheral blood mononuclear cells (PBMCs) inflammatory genes expression after long-term intervention
The venous blood samples will be taken for isolation of peripheral blood mononuclear cells (PBMCs). Extracted total RNA will then be used for running quantitative-PCR.
Time frame: -30 min. (Day-28 control); 180 min. (Day-28 control); -30 min. (Day-28 berry); 180 min. (Day-28 berry)
Postprandial selective attention after long-term intervention
The selective attention will be assessed using a standardized software (Röd and Grön software) that measures wrong/right decisions and time of reaction when the subjects is faced with a challenging stimulus.
Time frame: 45, 90, and 180 min after challenge meal.
Postprandial interleukin-6 (IL-6) after acute intervention
The venous blood will be collected for IL-6 analysis using ELISA commercial kits
Time frame: -30, 60, 120, and 180 min.
Incremental area under curve (iAUC) of interleukin-6 (IL-6) after long-term intervention
The venous blood will be collected for IL-6 analysis using ELISA commercial kits
Time frame: 4 weeks
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